Impact of age, body weight and metabolic risk factors on steroid reference intervals in men

Autor: Uberto Pagotto, Margherita Baccini, Alessia Fazzini, Flaminia Fanelli, Marco Mezzullo, Alessandra Gambineri, Carla Pelusi, Valentina Vicennati, Guido Di Dalmazi, Andrea Repaci
Přispěvatelé: Mezzullo M., Di Dalmazi G., Fazzini A., Baccini M., Repaci A., Gambineri A., Vicennati V., Pelusi C., Pagotto U., Fanelli F.
Rok vydání: 2020
Předmět:
Male
Hydrocortisone
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Body Mass Index
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Risk Factors
Tandem Mass Spectrometry
Corticosterone
Age Factor
Multivariate Analysi
Testosterone
2. Zero hunger
17-alpha-Hydroxyprogesterone
Age Factors
Dihydrotestosterone
General Medicine
030220 oncology & carcinogenesis
Human
medicine.drug
medicine.medical_specialty
Cortodoxone
Dehydroepiandrosterone
030209 endocrinology & metabolism
Estrone
03 medical and health sciences
Insulin resistance
Internal medicine
medicine
Humans
Obesity
Cross-Sectional Studie
business.industry
Risk Factor
Insulin
Body Weight
Androstenedione
Overweight
medicine.disease
Cross-Sectional Studies
chemistry
Multivariate Analysis
business
Chromatography
Liquid
Zdroj: European Journal of Endocrinology. 182:459-471
ISSN: 1479-683X
0804-4643
Popis: Objective To evaluate the independent impact of age, obesity and metabolic risk factors on 13 circulating steroid levels; to generate reference intervals for adult men. Design Cross-sectional study. Methods Three hundred and fifteen adults, drug-free and apparently healthy men underwent clinical and biochemical evaluation. Thirteen steroids were measured by LC-MS/MS and compared among men with increasing BMI. Moreover, the independent impact of age, BMI and metabolic parameters on steroid levels was estimated. Upper and lower reference limits were generated in steroid-specific reference sub-cohorts and compared with dysmetabolic sub-cohorts. Results We observed lower steroid precursors and testosterone and increase in estrone levels in men with higher BMI ranges. By multivariate analysis, 17-hydroxyprogesterone and dihydrotestosterone decreased with BMI, while cortisol decreased with waist circumference. Estrone increased with BMI and systolic blood pressure. Testosterone decreased with worsening insulin resistance. 17-hydroxypregnenolone and corticosterone decreased with increasing total/HDL-cholesterol ratio. Age-related reference intervals were estimated for 17-hydroxypregnenolone, DHEA, 17-hydroxyprogesterone, corticosterone, 11-deoxycortisol, cortisol and androstenedione, while age-independent reference intervals were estimated for progesterone, 11-deoxycorticosterone, testosterone, dihydrotestosterone, estrone and estradiol. Testosterone lower limit was 2.29 nmol/L lower (P = 0.007) in insulin resistant vs insulin sensitive men. Furthermore, the upper limits for dihydrotestosterone (−0.34 nmol/L, P = 0.045), cortisol (−87 nmol/L, P = 0.045–0.002) and corticosterone (−10.1 nmol/L, P = 0.048–0.016) were lower in overweight/obese, in abdominal obese and in dyslipidaemic subjects compared to reference sub-cohorts, respectively. Conclusions Obesity and mild unmedicated metabolic risk factors alter the circulating steroid profile and bias the estimation of reference limits for testosterone, dihydrotestosterone, cortisol and corticosterone. Applying age-dependent reference intervals is mandatory for steroid precursors and corticosteroids.
Databáze: OpenAIRE
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