Unravelling the Non-Native Low-Spin State of the Cytochrome c–Cardiolipin Complex: Evidence of the Formation of a His-Ligated Species Only
Autor: | Rebecca Pogni, Roberto Santucci, Maria Cristina Piro, Federica Sinibaldi, Barry D. Howes, Lisa Milazzo, Maria Camilla Baratto, Maria Fittipaldi, Lorenzo Tognaccini, Giulietta Smulevich |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Protein Folding Protein Structure Secondary Cardiolipins Stereochemistry Mutant Gene Expression 010402 general chemistry 01 natural sciences Biochemistry Protein Structure Secondary Dissociation (chemistry) law.invention 03 medical and health sciences chemistry.chemical_compound Methionine law Genes Synthetic Escherichia coli Cardiolipin Animals Histidine Horses Cloning Molecular Settore BIO/10 Electron paramagnetic resonance Conformational isomerism Protein Unfolding Carbon Monoxide biology Hydrogen bond Chemistry Myocardium Cytochrome c Synthetic Temperature Molecular Cytochromes c Hydrogen Bonding Recombinant Proteins 0104 chemical sciences 030104 developmental biology Genes Protein Binding biology.protein Cloning Peroxidase |
Zdroj: | Biochemistry. 56:1887-1898 |
ISSN: | 1520-4995 0006-2960 |
Popis: | The interaction between cytochrome c (Cyt c) and cardiolipin (CL) plays a vital role in the early stages of apoptosis. The binding of CL to Cyt c induces a considerable increase in its peroxidase activity that has been attributed to the partial unfolding of the protein, dissociation of the Met80 axial ligand, and formation of non-native conformers. Although the interaction between Cyt c and CL has been extensively studied, there is still no consensus regarding the conformational rearrangements of Cyt c that follow the protein-lipid interaction. To rationalize the different results and gain better insight into the Cyt c-CL interaction, we have studied the formation of the CL complex of the horse heart wild-type protein and selected mutants in which residues considered to play a key role in the interaction with CL (His26, His33, Lys72, Lys73, and Lys79) have been mutated. The analysis was conducted at both room temperature and low temperatures via ultraviolet-visible absorption, resonance Raman, and electron paramagnetic resonance spectroscopies. The trigger and the sequence of CL-induced structural variations are discussed in terms of disruption of the His26-Pro44 hydrogen bond. We unequivocally identify the sixth ligand in the partially unfolded, non-native low-spin state that Cyt c can adopt following the protein-lipid interaction, as a His ligation, ruling out the previously proposed involvement of a Lys residue or an OH- ion. |
Databáze: | OpenAIRE |
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