142 Nulliparity alters gene expression in inner cell mass and trophoblast of equine blastocysts in old mares
| Autor: | Catherine Archilla, L. Briot, Yan Jaszczyszyn, C. Gourtay, E. Derisoud, Luc Jouneau, Michèle Dahirel, Véronique Duranthon, F. De geoffroy, Pascale Chavatte-Palmer, Nathalie Peynot, A. Margat, Nathalie Daniel |
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| Přispěvatelé: | Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), École nationale vétérinaire - Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE), École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute for Integrative Biology of the Cell (I2BC), Institut Français du Cheval et de L'équitation (IFCE) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
blastocysts
Reproductive technology Nulliparity Biology Andrology Endocrinology Gene expression Genetics medicine Inner cell mass Molecular Biology Gene reproductive and urinary physiology 2. Zero hunger [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Trophoblast Embryo culture Embryo trophoblast Myosin complex medicine.anatomical_structure Reproductive Medicine embryonic structures Animal Science and Zoology Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development 47TH ANNUAL CONFERENCE OF THE IETS 47TH ANNUAL CONFERENCE OF THE IETS, Jan 2021, online, United States. pp.179, ⟨10.1071/RDv33n2Ab142⟩ |
| DOI: | 10.1071/RDv33n2Ab142⟩ |
| Popis: | An increased incidence in early embryo loss has been observed in aged mares. Moreover, the first foal born to a mare is lighter than her subsequent foals, with reported impaired placental function at term. Because trophoblast function may be affected from the embryo stage, the aim of this project was to determine the effect of parity in aged mares on gene expression in Day-8.5 embryos. Middle-aged (13.5±2.2 years) nulliparous (never foaled) (ON) or multiparous (1.8±1.6 foals) (OM) Saddlebred, non-nursing mares were inseminated with the semen of one unique stallion. At 8 days post-ovulation (10 days post-hCG), embryos were recovered by uterine flushing and bisected to obtain samples of pure (trophectoderm, TE) or inner cell mass enriched (ICM) trophoblast. Paired end, non-oriented RNA sequencing was performed with Illumina (NextSEqn 500) on 5 and 6 TE and ICM collected from ON and OM, respectively. Differential expression was analysed with DESEqn 2. Embryo size was included in the model and a P |
| Databáze: | OpenAIRE |
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