Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling

Autor:	Graeme Martin, César Cortez-Romero, Arturo Pro-Martínez, Carlos Miguel Becerril-Pérez, Jaime Gallegos-Sánchez, José Manuel Hernández-Hernández
Rok vydání:	2021
Předmět:	LH sheep medicine.medical_specialty Veterinary medicine medicine.medical_treatment media_common.quotation_subject Pulsatile flow Biology Article kisspeptin Kisspeptin anestrus Internal medicine SF600-1100 restricted suckling medicine Saline Ovulation media_common Estrous cycle General Veterinary Domestic sheep reproduction Endocrinology QL1-991 Animal Science and Zoology Pulsatile secretion Luteinizing hormone Zoology hormones hormone substitutes and hormone antagonists
Zdroj:	Animals Volume 11 Issue 9 Animals, Vol 11, Iss 2656, p 2656 (2021) Animals : an Open Access Journal from MDPI
ISSN:	2076-2615
DOI:	10.3390/ani11092656
Popis:	This study tested whether the intravenous application of kisspeptin can stimulate the pulsatile secretion of LH in suckling ewes during postpartum anestrus. Ten days after lambing, Pelibuey ewes were allocated among two groups: (1) continuous suckling (n = 8), where the lambs remained with their mothers and (2) restricted suckling (n = 8), where the mothers suckled their lambs twice daily for 30 min. On Day 19 postpartum, the ewes were individually penned with ad libitum access to water and feed and given an indwelling catheter in each jugular vein. On Day 20, 4 mL of blood was sampled every 15 min from 08:00 to 20:00 h to determine LH pulse frequency. At 14:00 h, four ewes in each group received 120 μg of kisspeptin diluted in 3 mL of saline as a continuous infusion for 6 h the remaining four ewes in each group received only saline. The interaction between kisspeptin and suckling type did not affect LH pulse frequency (p > 0.05). Before kisspeptin administration, pulse frequency was similar in all groups (1.50 ± 0.40 pulses per 6 h p > 0.05). With the application of kisspeptin, pulse frequency increased to 3.50 ± 0.43 pulses per 6 h (p ≤ 0.014), so the concentration of LH (1.11 ± 0.14 ng mL−1) was greater in kisspeptin-treated ewes than in saline-treated ewes (0.724 ± 0.07 ng mL−1 p ≤ 0.040). The frequency of LH pulses was greater with restricted suckling than with continuous suckling (2.44 ± 0.29 versus 1.69 ± 0.29 pulses per 6 h p ≤ 0.040). We conclude that intravenous application of kisspeptin increases the pulsatile secretion of LH in suckling ewes and that suckling might reduce kisspeptin neuronal activity, perhaps explaining the suppression of ovulation. Moreover, the effects of kisspeptin and suckling on pulsatile LH secretion appear to be independent, perhaps operating through different neural pathways.
Databáze:	OpenAIRE
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