Point mutation impairs centromeric CENH3 loading and induces haploid plants
| Autor: | Andreas Houben, Oda Weiss, Ali Mohammad Banaei-Moghaddam, Veit Schubert, Nils Stein, Jochen Kumlehn, Takayoshi Ishii, Kerstin Koch, Markus Niessen, Dmitri Demidov, Stefan Heckmann, Raheleh Karimi-Ashtiyani, Jörg Fuchs, Klaus M. Schmidt, Maia Gurushidze |
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| Rok vydání: | 2015 |
| Předmět: |
Genotype
Chromosomal Proteins Non-Histone Blotting Western Centromere Mutant Arabidopsis Haploidy Autoantigens Histones Histone H3 Point Mutation Kinetochores Plant Proteins Genetics Multidisciplinary Models Genetic biology Reverse Transcriptase Polymerase Chain Reaction Kinetochore Point mutation fungi food and beverages Chromosome Hordeum Plants Biological Sciences Plants Genetically Modified biology.organism_classification Chromatin Phenotype Microscopy Fluorescence Seeds Microscopy Electron Scanning Centromere Protein A |
| Zdroj: | Proceedings of the National Academy of Sciences. 112:11211-11216 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1504333112 |
| Popis: | The chromosomal position of the centromere-specific histone H3 variant CENH3 (also called "CENP-A") is the assembly site for the kinetochore complex of active centromeres. Any error in transcription, translation, modification, or incorporation can affect the ability to assemble intact CENH3 chromatin and can cause centromere inactivation [Allshire RC, Karpen GH (2008) Nat Rev Genet 9 (12):923-937]. Here we show that a single-point amino acid exchange in the centromere-targeting domain of CENH3 leads to reduced centromere loading of CENH3 in barley, sugar beet, and Arabidopsis thaliana. Haploids were obtained after cenh3 L130F-complemented cenh3-null mutant plants were crossed with wild-type A. thaliana. In contrast, in a noncompeting situation (i.e., centromeres possessing only mutated or only wild-type CENH3), no uniparental chromosome elimination occurs during early embryogenesis. The high degree of evolutionary conservation of the identified mutation site offers promising opportunities for application in a wide range of crop species in which haploid technology is of interest. |
| Databáze: | OpenAIRE |
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