Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen
| Autor: | Catalina Castañeira, L. Losinno, Carolina Natalia Alonso, Ana Flores Bragulat |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
endocrine system
food.ingredient Motility Semen Diluente de gema de ovo SF1-1100 law.invention Andrology food Semen preservation law Yolk SEMEN PRESERVATION Donkey Centrifugation DONKEY Sperm motility Seminal plasma Egg yolk extender General Veterinary SEMINAL PLASMA Cooled semen Chemistry urogenital system Sêmen resfriado Extender Jumento Sperm Preservação de sêmen Animal culture COOLED SEMEN Plasma seminal EGG YOLK EXTENDER purl.org/becyt/ford/4.3 [https] purl.org/becyt/ford/4 [https] |
| Zdroj: | Brazilian Journal of Veterinary Research and Animal Science, Vol 58 (2021) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Brazilian Journal of Veterinary Research and Animal Science; Vol. 58 (2021): Special Issue: Donkeys and Mules; e174301 Brazilian Journal of Veterinary Research and Animal Science; v. 58 (2021): Número especial: Asininos e Muares; e174301 Brazilian Journal of Veterinary Research and Animal Science; V. 58 (2021): Special Issue: Donkeys and Mules; e174301 Brazilian Journal of Veterinary Research and Animal Science Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
| ISSN: | 1678-4456 1413-9596 |
| Popis: | Developing effective cooled semen protocols is essential to increase pregnancy rates and reproductive efficiency in donkeys. This study aimed to evaluate the effect on sperm kinetic parameters and membrane integrity in cooled donkey semen diluted with defined milk proteins extender with 1% or 2% of egg yolk and the removal of seminal plasma. Twenty-four ejaculates from six jackasses were collected. Each ejaculate was divided into four aliquots that were diluted in extender with 1% (EY1) or 2% (EY2) egg yolk. One sample from each group was centrifuged, seminal plasma was removed (CEY1, CEY2 groups, respectively), and the samples were then refrigerated at 5 °C for 24 h. Fresh and cooled semen samples were assessed for sperm motility, morphology, and plasma membrane integrity. Total motility, progressive motility, sperm kinetic parameters, or live sperm cells were not statistically different when semen was cooled with an extender supplemented with 1% or 2% of egg yolk. Seminal plasma removal does not affect total motility or sperm kinetic parameters. However, progressive motility decreased (P |
| Databáze: | OpenAIRE |
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