A Cross-Linking Study on the Particle Species of Human Plasma High Density Lipoproteins
| Autor: | Youichi Yachida, Osamu Minari |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Apolipoprotein C
Chemical Phenomena Apolipoprotein B Biochemistry Gel permeation chromatography chemistry.chemical_compound High-density lipoprotein Centrifugation Density Gradient Humans Particle Size Molecular Biology Gel electrophoresis Chromatography biology General Medicine Lipids Chemistry Apolipoproteins Cross-Linking Reagents chemistry Chromatography Gel biology.protein Cholesteryl ester Particle lipids (amino acids peptides and proteins) Lipoproteins HDL Lipoprotein |
| Zdroj: | The Journal of Biochemistry. 94:459-471 |
| ISSN: | 1756-2651 0021-924X |
| DOI: | 10.1093/oxfordjournals.jbchem.a134376 |
| Popis: | The present investigation was on the particle species of human plasma high density lipoprotein (HDL) characterized by the stoichiometry of their apoprotein components. HDL2-1, HDL2-2, HDL3-1, and HDL3-2 isolated from normal human plasma by sequential ultracentrifugal flotation were further subfractionated by Bio Gel A-5m gel chromatography or hydroxyapatite column chromatography, and three distinct subfractions were obtained. Subfraction 1 was obtained from all the HDL fractions and it contained mostly apolipoprotein A-I (A-I). Subfraction 2 was obtained from HDL2-2 and HDL3-1 and it contained A-I and apolipoprotein A-II (A-II) in the molar ratio of one to one, and subfraction 3 from HDL2-2 and HDL3-1 contained A-I and apolipoprotein C (C). Each subfraction was treated with bifunctional cross-linking reagents, and the intraparticle cross-linked products of apolipoproteins were examined by SDS-polyacrylamide gel electrophoresis. The results of the cross-linking studies indicated that the HDL2 fraction consisted mainly of lipoprotein particles of the (A-I)4 type and a few of the (A-I)5, (A-I)2(A-II)2, and (A-I)4(C)2 types, and that the HDL3 fraction consisted mainly of (A-I)2(A-II)2 type particles and a few (A-I)4, (A-I)3, (A-I)2, (A-I), and (A-I)3(C)2 type particles. From the results of analyses of the lipid components in the HDL of each type, it was suggested that the function of the particle species of the (A-I)n type (n = 1--5), which contained more cholesteryl ester than the (A-I)2(A-II)2 type, was concerned mainly with cholesterol metabolism. |
| Databáze: | OpenAIRE |
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