Zinc supplementation ameliorates glycoprotein components and oxidative stress changes in the lung of streptozotocin diabetic rats

Autor: Ozgur Mutlu, Refiye Yanardag, Ozlem Sacan, Bertan Boran Bayrak, Nuriye Akev, Ismet Burcu Turkyilmaz
Rok vydání: 2015
Předmět:
0301 basic medicine
medicine.medical_specialty
Antioxidant
medicine.medical_treatment
chemistry.chemical_element
Zinc
medicine.disease_cause
General Biochemistry
Genetics and Molecular Biology
Streptozocin
Diabetes Mellitus
Experimental
Biomaterials
Superoxide dismutase
Lipid peroxidation
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
medicine
Animals
Xanthine oxidase
Lung
Carbonic Anhydrases
Peroxidase
Glutathione Peroxidase
biology
L-Lactate Dehydrogenase
Chemistry
Aryldialkylphosphatase
Superoxide Dismutase
Metals and Alloys
Paraoxonase
Glutathione
Catalase
Zinc Sulfate
Rats
Oxidative Stress
030104 developmental biology
Endocrinology
030220 oncology & carcinogenesis
Dietary Supplements
biology.protein
Female
Lipid Peroxidation
Sodium-Potassium-Exchanging ATPase
General Agricultural and Biological Sciences
Oxidative stress
Zdroj: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. 29(2)
ISSN: 1572-8773
Popis: Zinc (Zn) is a component of numerous enzymes that function in a wide range of biological process, including growth, development, immunity and intermediary metabolism. Zn may play a role in chronic states such as cardiovascular disease and diabetes mellitus. Zn acts as cofactor and for many enzymes and proteins and has antioxidant, antiinflammatory and antiapoptotic effects. Taking into consideration that lung is a possible target organ for diabetic complications, the aim of this study was to investigate the protective role of zinc on the glycoprotein content and antioxidant enzyme activities of streptozotocin (STZ) induced diabetic rat tissues. Female Swiss albino rats were divided into four groups. Group I, control; Group II, control + zinc sulfate; Group III, STZ-diabetic; Group IV, diabetic + zinc sulfate. Diabetes was induced by intraperitoneal injection of STZ (65 mg/kg body weight). Zinc sulfate was given daily by gavage at a dose of 100 mg/kg body weight every day for 60 days to groups II and IV. At the last day of the experiment, rats were sacrificed, lung tissues were taken. Also, glycoprotein components, tissue factor (TF) activity, protein carbonyl (PC), advanced oxidative protein products (AOPP), hydroxyproline, and enzyme activities in lung tissues were determined. Glycoprotein components, TF activity, lipid peroxidation, non enzymatic glycation, PC, AOPP, hydroxyl proline, lactate dehydrogenase, catalase, superoxide dismutase, myeloperoxidase, xanthine oxidase, adenosine deaminase and prolidase significantly increased in lung tissues of diabetic rats. Also, glutathione levels, paraoxonase, arylesterase, carbonic anhydrase, and Na(+)/K(+)- ATPase activities were decreased. Administration of zinc significantly reversed these effects. Thus, the study indicates that zinc possesses a significantly beneficial effect on the glycoprotein components and oxidant/antioxidant enzyme activities.
Databáze: OpenAIRE
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