Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion
| Autor: | Renato A. Mortara, Silvia Y. Kawashita, Claudio Vieira da Silva, Samuel Goldenberg, Bruno Dallagiovanna, Thaïs Souto-Padrón, Marcelo R. S. Briones, Mario Cruz, Marco Aurélio Krieger, Christian Probst, Erika Alves da Silva, Norma W. Andrews |
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| Rok vydání: | 2009 |
| Předmět: |
Virulence Factors
Trypanosoma cruzi media_common.quotation_subject Molecular Sequence Data Immunology Cell Protozoan Proteins Biology Microbiology law.invention law Chlorocebus aethiops medicine Extracellular Animals Humans Internalization Amastigote Vero Cells media_common Sequence Analysis DNA DNA Protozoan biology.organism_classification Molecular biology Molecular Weight Infectious Diseases medicine.anatomical_structure Polyclonal antibodies Recombinant DNA biology.protein Antibody HeLa Cells Protein Binding |
| Zdroj: | Microbes and Infection. 11:563-570 |
| ISSN: | 1286-4579 |
| DOI: | 10.1016/j.micinf.2009.03.007 |
| Popis: | Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence that codes for a 21 kDa protein that showed high probability of being secreted was selected. After cloning this protein sequence, the results showed that it was a ubiquitous protein and secreted by extracellular amastigotes. The recombinant form (P21-His6) adhered to HeLa cells in a dose-dependent manner. Pretreatment of host cells with P21-His6 inhibited cell invasion by extracellular amastigotes from G and CL strains. On the other hand, when the protein was added to host cells at the same time as amastigotes, an increase in cell invasion was observed. Host-cell pretreatment with P21-His6 augmented invasion by metacyclic trypomastigotes. Moreover, polyclonal antibody anti-P21 inhibited invasion only by extracellular amastigotes and metacyclic trypomastigotes from G strain. These results suggested that P21 might be involved in T. cruzi cell invasion. We hypothesize that P21 could be secreted in the juxtaposition parasite-host cell and triggers signaling events yet unknown that lead to parasite internalization. 2009 Elsevier Masson SAS. All rights reserved. |
| Databáze: | OpenAIRE |
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