Dynamic and differential Oct-1 expression during early Xenopus embryogenesis: persistence of Oct-1 protein following down-regulation of the RNA
| Autor: | Peter C. van der Vliet, Henk A. Karg, Gert Jan C. Veenstra, Olivier Destrée, Joselin Peterson-Maduro, Tim L. Beumer, Brenda I. Stegeman |
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| Rok vydání: | 1995 |
| Předmět: |
Embryology
Embryo Nonmammalian genetic structures Xenopus Down-Regulation Xenopus Proteins Biology Embryomics Cell Line Xenopus laevis Ectoderm Animals Gene Transcription factor Antibodies Monoclonal Gene Expression Regulation Developmental Neural crest RNA Embryo biology.organism_classification Molecular biology eye diseases DNA-Binding Proteins embryonic structures Homeobox sense organs Host Cell Factor C1 Cell Division Octamer Transcription Factor-1 Transcription Factors Developmental Biology |
| Zdroj: | Mechanisms of Development. 50:103-117 |
| ISSN: | 0925-4773 |
| DOI: | 10.1016/0925-4773(94)00328-k |
| Popis: | As a first step towards the elucidation of the role of the transcription factor Oct-1 in development, we prepared a monoclonal antibody to study the spatio-temporal distribution of Oct-1 protein in vivo. Here we report differential expression of the Oct-1 gene in the Xenopus embryo both at the RNA and the protein level. Transcripts and protein are detected in ectodermal and mesodermal cell lineages, in which the expression exhibits a pattern of progressive spatial restriction in the course of development. The Oct-1 expression as reported here is not correlated with cell density or cell proliferation in the embryo. Our results suggest a role of Oct-1 in the specification and differentiation of neuronal and neural crest cells. In many other cells, the developmental decision to down regulate Oct-1 is delayed, probably due to a high stability of the protein. |
| Databáze: | OpenAIRE |
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