A multi-responsive coumarin–benzothiazole fluorescent probe for selective detection of biological thiols and hydrazine
| Autor: | Yanbo Li, Heping Li, Kangni Chen, Jinyan Shang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
QD71-142
Chemistry Biological thiols Hydrazine General Physics and Astronomy General Chemistry Coumarin Combinatorial chemistry Fluorescence General Biochemistry Genetics and Molecular Biology Fluorescent probe chemistry.chemical_compound Benzothiazole General Materials Science Binding site Hydrate Selectivity Acetonitrile QD1-999 Analytical chemistry General Environmental Science |
| Zdroj: | Journal of Analytical Science and Technology, Vol 12, Iss 1, Pp 1-12 (2021) |
| ISSN: | 2093-3371 |
| DOI: | 10.1186/s40543-021-00302-5 |
| Popis: | Biothiols play important roles in various physiological and biological processes, which closely related to many diseases. Hydrazine is widely used in the chemical industry, but it is harmful to humans and animals. Therefore, it is very important to develop a fluorescent probe that can simultaneously detect biological thiols and hydrazine. In this work, a new fluorescent probe (2E,4Z)-2-(benzo[d]thiazol-2-yl)-5-chloro-5-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)penta-2,4-dienenitrile (BCD) was synthesized by integrating coumarin and benzothiazole acetonitrile. Featured with four binding sites and different bonding mechanism between probe with biothiols and hydrazine, this probe exhibited fluorescent turn-on for distinguishing Cys, Hcy, GSH and hydrazine with 760-, 8-, 6- and 637-fold fluorescent intensity increase at 502, 479, 476 and 458 nm, respectively, through different excitation wavelengths. Research on the effect of pH on the fluorescent performance of BCD shows that the probe exhibits superior stability in a weakly alkaline to weakly acidic environment, which will facilitate the detection of biological thiols or hydrazine hydrate by BCD. Selectivity studies have shown that the probe has high specificity to biological thiols and hydrazine, which is of great significance to the application of BCD. |
| Databáze: | OpenAIRE |
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