Leishmania mexicana promastigotes down regulate JNK and p-38 MAPK activation: Role in the inhibition of camptothecin-induced apoptosis of monocyte-derived dendritic cells
Autor: | M. Magdalena Aguirre-García, Arturo A. Wilkins-Rodríguez, Jesús Argueta-Donohué, Jorge Rodríguez-González, Laila Gutiérrez-Kobeh |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
MAPK/ERK pathway MAP Kinase Kinase 4 p38 mitogen-activated protein kinases Leishmania mexicana Immunology Down-Regulation Apoptosis p38 Mitogen-Activated Protein Kinases Mice 03 medical and health sciences 0302 clinical medicine parasitic diseases In Situ Nick-End Labeling Animals Humans Phosphorylation Cells Cultured Mice Inbred BALB C biology Kinase Macrophages Dendritic Cells General Medicine Leishmania biology.organism_classification Antineoplastic Agents Phytogenic Cell biology 030104 developmental biology Infectious Diseases 030220 oncology & carcinogenesis Mitogen-activated protein kinase biology.protein DNA fragmentation Camptothecin Female Parasitology |
Zdroj: | Experimental Parasitology. 163:57-67 |
ISSN: | 0014-4894 |
Popis: | Dendritic cells (DC) are one of the principal host cells of the obligate intracellular parasite Leishmania. Inhibition of host cell apoptosis is a strategy employed by multiple pathogens to ensure their survival in the infected cell. We have previously shown that the infection of monocyte-derived dendritic cells (moDC) with Leishmania mexicana inhibits campthotecin-induced apoptosis. Nevertheless, the mechanisms involved in the inhibition of apoptosis of dendritic cells by Leishmania have not been established. Mitogen-activated protein kinases (MAPK) are key participants in the process of apoptosis and different species of Leishmania have been shown to regulate these kinases. In the present study, we analyzed the effect of L. mexicana promastigotes in the activation of JNK and p38 MAP kinase and their participation in the inhibition of apoptosis. The infection of moDC with L. mexicana promastigotes diminished significantly the phosphorylation of the MAP kinases JNK and p38. The inhibition of both kinases diminished DNA fragmentation, but in a major extent was the reduction of DNA fragmentation when JNK was inhibited. The capacity of L. mexicana promastigotes to diminish MAP kinases activation is probably one of the strategies employed to delay apoptosis induction in the infected moDC and may have implications for Leishmania pathogenesis by favoring the invasion of its host and the persistence of the parasite in the infected cells. |
Databáze: | OpenAIRE |
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