Use of Alkaline Phosphatase Staining to Differentiate Canine Osteosarcoma from Other Vimentin-positive Tumors
| Autor: | Walter E. Hoffmann, L. P. De Lorimier, Anne M. Barger, Timothy M. Fan, Joanne B. Messick, K. Bailey, R. Graca |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Pathology medicine.medical_specialty Indoles 040301 veterinary sciences Bone Neoplasms Vimentin Biology Sensitivity and Specificity 0403 veterinary science 03 medical and health sciences Dogs medicine Animals Dog Diseases Coloring Agents Fibrosarcoma Osteosarcoma Staining and Labeling General Veterinary Nitroblue Tetrazolium 04 agricultural and veterinary sciences Alkaline Phosphatase medicine.disease Immunohistochemistry Staining 030104 developmental biology Primary bone biology.protein Alkaline phosphatase Sarcoma Chondrosarcoma |
| Zdroj: | Veterinary Pathology. 42:161-165 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1354/vp.42-2-161 |
| Popis: | Aspiration of lytic bone lesions is an excellent diagnostic test in the initial evaluation of primary bone neoplasia. However, cytologically, it can be difficult to differentiate osteosarcoma (OSA) from other bone neoplasms, including fibrosarcoma, chondrosarcoma, synovial cell sarcoma, and plasma cell myeloma. The purpose of this study is to determine the sensitivity and specificity of alkaline phosphatase (ALP) staining to differentiate OSA from other tumors that express vimentin by immunocytochemistry or immunohistochemistry. ALP is a hydrolytic enzyme present in multiple tissues including liver, kidney, intestine, placenta, and bone. Hypothetically, neoplasms actively producing bone should be specifically positive for ALP staining. Unstained, cytologic specimens were incubated for 8-10 minutes with nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate toluidine salt-phosphatase substrate. A positive reaction stains the membrane of the cells gray to black. Samples were counterstained with a Romanowsky's stain to determine whether the sample was of representative cellularity. A total of 61 vimentin-positive neoplasms have been evaluated and confirmed histopathologically. Tumors that expressed vimentin and were positive for ALP included 33 OSAs, one multilobular tumor of bone, one amelanotic melanoma, and one chondrosarcoma. Tumors that expressed vimentin and were negative for ALP included chondrosarcomas (three of four), multiple fibrosarcomas, and multiple synovial cell sarcomas. The sensitivity is 100%, and the specificity is 89%. In conclusion, ALP appears to be a highly sensitive and fairly specific marker in the diagnosis of OSA. |
| Databáze: | OpenAIRE |
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