Three-dimensional reconstruction of systematic histological sections: application to observations on palatal shelf elevation
| Autor: | Xiaoming Wang, Jing Zhang, Yinuo Wang, Chenghao Li, Bing Shi, Yahong Wang, Weilong Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Secondary palate development Article 03 medical and health sciences 0302 clinical medicine Imaging Three-Dimensional Tongue Developmental biology medicine Genetics Animals General Dentistry Process (anatomy) Palate 3D reconstruction Elevation RK1-715 030206 dentistry Anatomy Sagittal plane Cleft Palate 030104 developmental biology medicine.anatomical_structure Coronal plane Dentistry Morphological analysis Geology |
| Zdroj: | International Journal of Oral Science International Journal of Oral Science, Vol 13, Iss 1, Pp 1-7 (2021) |
| ISSN: | 2049-3169 1674-2818 |
| Popis: | Normal mammalian secondary palate development undergoes a series of processes, including palatal shelf (PS) growth, elevation, adhesion and fusion, and palatal bone formation. It has been estimated that more than 90% of isolated cleft palate is caused by defects associated with the elevation process. However, because of the rapidly completed elevation process, the entire process of elevation will never be easy to clarify. In this article, we present a novel method for three-dimensional (3D) reconstruction of thick tissue blocks from two-dimensional (2D) histological sections. We established multiplanar sections of the palate and tongue in coronal and sagittal directions, and further performed 3D reconstruction to observe the morphological interaction and connection between the two components prior to and during elevation. The method completes an imaging system for simultaneous morphological analysis of thick tissue samples using both synthetic and real data. The new method will provide a comprehensive picture of reorientation morphology and gene expression pattern during the palatal elevation process. |
| Databáze: | OpenAIRE |
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