First steps of bacteriophage SPP1 entry into Bacillus subtilis

Autor: Carlos São-José, Rut Carballido-López, Lina Jakutytė, Paulo Tavares, Rudi Lurz, Rimantas Daugelavičius, Catarina Baptista
Přispěvatelé: UPR3296, Unite Virol Mol & Struct, IFR 115, Centre National de la Recherche Scientifique (CNRS), Dept Biochem & Biophys, Vilnius University [Vilnius], Fac Farm, Ctr Patogenese Mol, Unidade Retrovirus & Infeccoes Associadas, Universidade de Lisboa (ULISBOA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Dept Biochem & Biotechnol, Vytautas Magnus Univ, University of Vilnius, Universite Paris-Sud, French embassy in Lithuania, program Egide Gilibert, European Union, Region Ile-de-France (SETCI), Fundacao para a Ciencia e a Tecnologia (FCT, MCTES, Portugal) [SFRH/BPD/34892/2007, PTDC/BIA-MIC/66412/2006], CNRS, INRA, Research Council of Lithuania [MIP-128/2010], [IFR115], University of Lisbon
Rok vydání: 2012
Předmět:
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Bacillus Phages
Bacillus subtilis
medicine.disease_cause
Membrane Potentials
Calcium Chloride
Virus entry
chemistry.chemical_compound
COLI CYTOPLASMIC MEMBRANE
chemistry.chemical_classification
Membrane potential
0303 health sciences
Depolarization
Bacteriophage SPP1
GHOST-INFECTED CELLS
3. Good health
Ca2+ ions
Biochemistry
ESCHERICHIA-COLI
TEICHOIC-ACIDS
YueB
GRAM-POSITIVE BACTERIA
Gram-positive bacterium
Virus Attachment
Genome
Viral
Biology
Permeability
CALCIUM
Divalent
03 medical and health sciences
Viral entry
Virology
ION CHANNELS
medicine
Escherichia coli
RECEPTOR YUEB
PHAGE DNA TRANSPORT
030304 developmental biology
DEOXYRIBONUCLEIC ACID
030306 microbiology
Cell Membrane
Virus Internalization
biology.organism_classification
Microscopy
Fluorescence
chemistry
Cytoplasm
Biophysics
Membrane voltage
DNA
Zdroj: Virology
Virology, Elsevier, 2012, 422 (2), pp.425-434. ⟨10.1016/j.virol.2011.11.010⟩
ISSN: 0042-6822
1096-0341
DOI: 10.1016/j.virol.2011.11.010
Popis: The mechanism of genome transfer from the virion to the host cytoplasm is critical to understand and control the beginning of viral infection. The initial steps of bacteriophage SPP1 infection of the Gram-positive bacterium Bacillus subtilis were monitored by following changes in permeability of the cytoplasmic membrane (CM). SPP1 leads to a distinctively faster CM depolarization than the one caused by podovirus phi 29 or myovirus SP01 during B. subtilis infection. Depolarization requires interaction of SPP1 infective virion to its receptor protein YueB. The amplitude of depolarization depends on phage input and concentration of YueB at the cell surface. Sub-millimolar concentrations of Ca2+ are necessary and sufficient for SPP1 reversible binding to the host envelope and thus to trigger depolarization while DNA delivery to the cytoplasm depends on millimolar concentrations of this divalent cation. A model describing the early events of bacteriophage SPP1 infection is presented. (C) 2011 Elsevier Inc. All rights reserved.
Databáze: OpenAIRE
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