Two Atypical Enteropathogenic Escherichia coli Strains Induce the Production of Secreted and Membrane-Bound Mucins To Benefit Their Own Growth at the Apical Surface of Human Mucin-Secreting Intestinal HT29-MTX Cells
| Autor: | Vanessa Liévin-Le Moal, Antonio José Piantino Ferreira, Monica Aparecida Midoli Vieira, Tânia A. T. Gomes, Terezinha Knöbl, Alain L. Servin |
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| Rok vydání: | 2010 |
| Předmět: |
Brush border
Immunology Virulence medicine.disease_cause digestive system Microbiology Bacterial Adhesion Cell Line Cell membrane Enteropathogenic Escherichia coli Ileum medicine Animals Humans Secretion Escherichia coli Escherichia coli Infections Cellular Microbiology: Pathogen-Host Cell Molecular Interactions biology Gene Expression Profiling Mucin Mucins biology.organism_classification Enterobacteriaceae Up-Regulation Disease Models Animal Enterocytes Infectious Diseases medicine.anatomical_structure Gene Expression Regulation Host-Pathogen Interactions Parasitology Rabbits |
| Zdroj: | Infection and Immunity. 78:927-938 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | In rabbit ligated ileal loops, two atypical enteropathogenic Escherichia coli (aEPEC) strains, 3991-1 and 0421-1, intimately associated with the cell membrane, forming the characteristic EPEC attachment and effacement lesion of the brush border, induced a mucous hypersecretion, whereas typical EPEC (tEPEC) strain E2348/69 did not. Using cultured human mucin-secreting intestinal HT29-MTX cells, we demonstrate that apically aEPEC infection is followed by increased production of secreted MUC2 and MUC5AC mucins and membrane-bound MUC3 and MUC4 mucins. The transcription of the MUC5AC and MUC4 genes was transiently upregulated after aEPEC infection. We provide evidence that the apically adhering aEPEC cells exploit the mucins' increased production since they grew in the presence of membrane-bound mucins, whereas tEPEC did not. The data described herein report a putative new virulence phenomenon in aEPEC. |
| Databáze: | OpenAIRE |
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