Expression analysis and functional characterization of the monosaccharide transporters, OsTMTs , involving vacuolar sugar transport in rice ( Oryza sativa )
| Autor: | Nayeon Ryoo, Tae-Ryong Hahn, H. Ekkehard Neuhaus, Bo Burla, Man-Ho Cho, Joon-Seob Eom, Dae-Woo Lee, Sang-Bong Choi, Hyun-Bi Kim, Soon-Kwan Hong, Enrico Martinoia, Jung-Il Cho, Jong-Seong Jeon, Seong Hee Bhoo |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Physiology
Recombinant Fusion Proteins Green Fluorescent Proteins Molecular Sequence Data Arabidopsis Plant Science Vacuole Gene Expression Regulation Plant Sugar transporter Cloning Molecular Promoter Regions Genetic Phylogeny Glucuronidase Plant Proteins Oryza sativa biology Genetic Complementation Test Membrane Transport Proteins food and beverages Biological Transport Oryza Plants Genetically Modified biology.organism_classification Subcellular localization Vascular bundle Genetically modified rice Glucose Biochemistry Organ Specificity Vacuoles Carbohydrate Metabolism Phloem Subcellular Fractions |
| Zdroj: | New Phytologist. 186:657-668 |
| ISSN: | 1469-8137 0028-646X |
| DOI: | 10.1111/j.1469-8137.2010.03194.x |
| Popis: | In Arabidopsis, the compartmentation of sugars into vacuoles is known to be facilitated by sugar transporters. However, vacuolar sugar transporters have not been studied in detail in other plant species. To characterize the rice (Oryza sativa) tonoplast monosaccharide transporters, OsTMT1 and OsTMT2, we analysed their subcellular localization using green fluorescent protein (GFP) and expression patterns using reverse-transcription polymerase chain reaction (RT-PCR), performed histochemical beta-glucuronidase (GUS) assay and in situ hybridization analysis, and assessed sugar transport ability using isolated vacuoles. Expression of OsTMT-GFP fusion protein in rice and Arabidopsis revealed that the OsTMTs localize at the tonoplast. Analyses of OsTMT promoter-GUS transgenic rice indicated that OsTMT1 and OsTMT2 are highly expressed in bundle sheath cells, and in vascular parenchyma and companion cells in leaves, respectively. Both genes were found to be preferentially expressed in the vascular tissues of roots, the palea/lemma of spikelets, and in the main vascular tissues and nucellar projections on the dorsal side of the seed coats. Glucose uptake studies using vacuoles isolated from transgenic mutant Arabidopsis (tmt1-2-3) expressing OsTMT1 demonstrated that OsTMTs are capable of transporting glucose into vacuoles. Based on expression analysis and functional characterization, our present findings suggest that the OsTMTs play a role in vacuolar glucose storage in rice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text