Mycobacterium tuberculosis CwsA overproduction modulates cell division and cell wall synthesis
Autor: | Malini Rajagopalan, Krishna Sarva, Murty V. V. S. Madiraju, Renata Plocinska, Przemysław Płociński, Luis Martinez |
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Rok vydání: | 2013 |
Předmět: |
Microbiology (medical)
Cell division Immunoblotting Mycobacterium smegmatis Immunology Biology Microbiology Cell wall Mycobacterium tuberculosis Bacterial Proteins Cell Wall Protein Interaction Mapping Escherichia coli Humans Overproduction FtsZ Cells Cultured Membrane Proteins Gene Expression Regulation Bacterial biology.organism_classification Fusion protein Anti-Bacterial Agents Up-Regulation Cell biology Infectious Diseases Membrane protein Isotope Labeling biology.protein Cell Division Protein Binding Mycobacterium |
Zdroj: | ResearcherID |
ISSN: | 1472-9792 |
Popis: | We recently showed that two small membrane proteins of Mycobacterium tuberculosis, CwsA and CrgA, interact with each other, and that loss of CwsA in M. smegmatis is associated with defects in the cell division and cell wall synthesis processes. Here we show that CwsA overproduction also affected growth, cell division and cell shape of M. smegmatis and M. tuberculosis. CwsA overproduction in M. tuberculosis led to increased sensitivity to cefsulodin, a penicillin-binding protein (PBP) 1A/1B targeting beta (β) -lactam, but was unaffected by other β-lactams and vancomycin. A M. smegmatis cwsA overexpressing strain showed bulgy cells, increased fluorescent vancomycin staining and altered localization of Wag31-mCherry fusion protein. However, the levels of phosphorylated Wag31, important for optimal peptidoglycan synthesis and growth in mycobacteria, were not affected. Interestingly, CwsA overproduction in E. coli led to the formation of large rounded cells that eventually lysed whereas the overproduction of FtsZ along with CwsA reversed this phenotype. Together, our results emphasize that optimal levels of CwsA are required for regulated cell wall synthesis, hence maintenance of cell shape, and that CwsA likely interacts with and modulates the activities of other cell wall synthetic components including PBPs. |
Databáze: | OpenAIRE |
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