Alteration of lymphocyte membrane phospholipids and intracellular free calcium concentrations in hyperlipidemic subjects
| Autor: | Attila Mohácsi, Ildikó Seres, Josiane Simon, M. Freyss-Beguin, Bertalan Kozlovszky, Tamas Fulop, Marie-Aude Devynck |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Intracellular Fluid
Male medicine.medical_specialty Membrane lipids Hypercholesterolemia Phospholipid chemistry.chemical_element Biology Calcium Membrane Lipids chemistry.chemical_compound Internal medicine medicine Humans Lymphocytes Phospholipids chemistry.chemical_classification Cholesterol Fatty Acids Hypertriglyceridemia Fatty acid Middle Aged medicine.disease Spectrometry Fluorescence Endocrinology chemistry Biochemistry Female lipids (amino acids peptides and proteins) Cardiology and Cardiovascular Medicine Intracellular Thymidine Polyunsaturated fatty acid |
| Zdroj: | Atherosclerosis. 121:175-183 |
| ISSN: | 0021-9150 |
| DOI: | 10.1016/0021-9150(95)05714-5 |
| Popis: | Hypercholesterolemia has been proposed to influence cell functions via changes in membrane composition. The aim of the present study was to determine whether the membrane phospholipid composition of human lymphocytes is modified in hypercholesterolemia and whether these changes are accompanied by functional modifications. The phospholipid fatty acid contents and intracellular free calcium concentrations were determined in peripheral blood lymphocytes from 13 subjects with serum total cholesterol levels ranging from 4.6 to 8.8 mmol/l. The spontaneous basal rate of thymidine incorporation in lymphocyte of hypercholesterolemic individuals increased, while its relative stimulation by ConA was less effective. Important changes in membrane lipid composition, consisting mainly of decrease of the mass of phospholipids, and of associated polyunsaturated fatty acids were observed in hypercholesterolemia. In contrast, the cell cholesterol content was significantly increased. The intracellular free calcium concentration was enhanced and strongly associated with circulating cholesterol levels, cell cholesterol content and phospholipid fatty acids. These results indicate that hypercholesterolemia is accompanied by profound changes in lymphocyte membrane lipid composition and Ca 2+ handling. |
| Databáze: | OpenAIRE |
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