Human–Soybean Allergies: Elucidation of the Seed Proteome and Comprehensive Protein–Protein Interaction Prediction
| Autor: | Bradley Barnes, Julia Hooker, Michael Sadowski, Bahram Samanfar, Hiroyuki Aoki, Ashkan Golshani, Kevin Dick, Arezo Pattang, Mohan Babu, Frank Dehne, Elroy R. Cober, Daniel Burnside, Sadhna Phanse, Nour Nissan, James R. Green, Le Hoa Tan |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of Proteome Research. 20:4925-4947 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/acs.jproteome.1c00138 |
| Popis: | The soybean crop, Glycine max (L.) Merr., is consumed by humans, Homo sapiens, worldwide. While the respective bodies of literature and -omics data for each of these organisms are extensive, comparatively few studies investigate the molecular biological processes occurring between the two. We are interested in elucidating the network of protein-protein interactions (PPIs) involved in human-soybean allergies. To this end, we leverage state-of-the-art sequence-based PPI predictors amenable to predicting the enormous comprehensive interactome between human and soybean. A network-based analytical approach is proposed, leveraging similar interaction profiles to identify candidate allergens and proteins involved in the allergy response. Interestingly, the predicted interactome can be explored from two complementary perspectives: which soybean proteins are predicted to interact with specific human proteins and which human proteins are predicted to interact with specific soybean proteins. A total of eight proteins (six specific to the human proteome and two to the soy proteome) have been identified and supported by the literature to be involved in human health, specifically related to immunological and neurological pathways. This study, beyond generating the most comprehensive human-soybean interactome to date, elucidated a soybean seed interactome and identified several proteins putatively consequential to human health. |
| Databáze: | OpenAIRE |
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