A transposon-derived small RNA regulates gene expression in Salmonella Typhimurium
| Autor: | Onella Charles, Ryan S. Trussler, David B. Haniford, Michael J. Ellis |
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| Rok vydání: | 2017 |
| Předmět: |
Salmonella typhimurium
0301 basic medicine Transposable element Small RNA Down-Regulation Biology 03 medical and health sciences Bacterial Proteins Gene expression Genetics RNA Messenger Gene Transposase Regulation of gene expression Base Sequence Sequence Analysis RNA Effector Gene Expression Profiling Gene Expression Regulation Bacterial Pathogenicity island Cell biology RNA Bacterial 030104 developmental biology Genes Bacterial DNA Transposable Elements RNA |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Bacterial sRNAs play an important role in regulating many cellular processes including metabolism, outer membrane homeostasis and virulence. Although sRNAs were initially found in intergenic regions, there is emerging evidence that protein coding regions of the genome are a rich reservoir of sRNAs. Here we report that the 5΄UTR of IS200 transposase mRNA (tnpA) is processed to produce regulatory RNAs that affect expression of over 70 genes in Salmonella Typhimurium. We provide evidence that the tnpA derived sRNA base-pairs with invF mRNA to repress expression. As InvF is a transcriptional activator of SPI-1 encoded and other effector proteins, tnpA indirectly represses these genes. We show that deletion of IS200 elements in S. Typhimurium increases invasion in vitro and reduces growth rate, while over-expression of tnpA suppresses invasion. Our work indicates that tnpA acts as an sRNA ‘sponge’ that sets a threshold for activation of Salmonella pathogenicity island (SPI)-1 effector proteins and identifies a new class of ‘passenger gene’ for bacterial transposons, providing the first example of a bacterial transposon producing a regulatory RNA that controls host gene expression. |
| Databáze: | OpenAIRE |
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