ExcessiveO-GlcNAcylation of proteins suppresses spontaneous cardiogenesis in ES cells
| Autor: | Jin Won Cho, Hoe Suk Kim, Woo Kyung Moon, Hyun Jung Joo, Jeong Gu Kang, Sang Yoon Park, Yu Rim Choi |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Phenylcarbamates Biophysics Biology Biochemistry Streptozocin Acetylglucosamine Cell Line Mice chemistry.chemical_compound O-GlcNAcylation Structural Biology Glucosamine Internal medicine Diabetic cardiomyopathy Acetylglucosaminidase Oximes O-linked N-acetylglucosamine Gene expression Genetics medicine Animals Humans Nkx2.5 transcription factor Gene Knock-In Techniques Molecular Biology Embryonic Stem Cells Glutamine amidotransferase Cardiogenesis Myocardium Diabetes Infant Cell Differentiation Heart Cell Biology medicine.disease Streptozotocin Embryonic stem cell Cell biology Glutamine Endocrinology chemistry Cell culture Female medicine.drug |
| Zdroj: | FEBS Letters. 583:2474-2478 |
| ISSN: | 0014-5793 |
| Popis: | Increased modification of proteins with O-linked N-acetylglucosamine (O-GlcNAc) has been implicated in the development of diabetic cardiomyopathy. We used the well-characterized ES cells (Nkx2.5GFP knock-in ES cells), to investigate the role of O-GlcNAcylation in cardiomyocyte development. O-GlcNAcylation decreased in differentiating ES cells, as did the expression of O-GlcNAc transferase. Increasing O-GlcNAcylation with glucosamine or by inhibiting N-acetylglucosaminidase (streptozotocin or PUGNAc) decreased the number of cardiomyocyte precursors and cardiac-specific gene expression. On the other hand, decreasing O-GlcNAcylation with an inhibitor of glutamine fructose-6-phosphate amidotransferase (6-diazo-5-oxo-norleucine) increased cardiomyocyte precursors. These results suggest that excessive O-GlcNAcylation impairs cardiac cell differentiation in ES cells. |
| Databáze: | OpenAIRE |
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