Periarteriolar Glioblastoma Stem Cell Niches Express Bone Marrow Hematopoietic Stem Cell Niche Proteins
Autor: | Wikky Tigchelaar, Hala Kakar, Mohammed Khurshed, Remco J. Molenaar, Britt van der Swaan, Jill R Wormer, Cornelis J.F. Van Noorden, Zbynek Tonar, Vashendriya V V Hira, Barbara Breznik, Renske Hulsbos |
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Přispěvatelé: | CCA - Cancer biology and immunology, Graduate School, Other departments, Amsterdam Gastroenterology Endocrinology Metabolism, Medical Biology |
Rok vydání: | 2018 |
Předmět: |
Adult
0301 basic medicine endocrine system Receptors CXCR4 Histology Stromal cell Hematopoietic stem cell niche Cathepsin K Niche CXCR4 03 medical and health sciences 0302 clinical medicine medicine Humans Stem Cell Niche Aged Staining and Labeling biology Brain Neoplasms CD44 Hematopoietic stem cell Articles Middle Aged Hematopoietic Stem Cells Immunohistochemistry Chemokine CXCL12 Cell biology Arterioles Hyaluronan Receptors 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Neoplastic Stem Cells biology.protein Osteopontin Bone marrow Anatomy Stem cell Glioblastoma |
Zdroj: | journal of histochemistry and cytochemistry, 66(3), 155-173. Histochemical Society Inc. |
ISSN: | 1551-5044 0022-1554 |
Popis: | In glioblastoma, a fraction of malignant cells consists of therapy-resistant glioblastoma stem cells (GSCs) residing in protective niches that recapitulate hematopoietic stem cell (HSC) niches in bone marrow. We have previously shown that HSC niche proteins stromal cell–derived factor-1α (SDF-1α), C-X-C chemokine receptor type 4 (CXCR4), osteopontin (OPN), and cathepsin K (CatK) are expressed in hypoxic GSC niches around arterioles in five human glioblastoma samples. In HSC niches, HSCs are retained by binding of SDF-1α and OPN to their receptors CXCR4 and CD44, respectively. Protease CatK cleaves SDF-1α to release HSCs out of niches. The aim of the present study was to reproduce the immunohistochemical localization of these GSC markers in 16 human glioblastoma samples with the addition of three novel markers. Furthermore, we assessed the type of blood vessels associated with GSC niches. In total, we found seven GSC niches containing CD133-positive and nestin-positive GSCs as a single-cell layer exclusively around the tunica adventitia of 2% of the CD31-positive and SMA-positive arterioles and not around capillaries and venules. Niches expressed SDF-1α, CXCR4, CatK, OPN, CD44, hypoxia-inducible factor-1α, and vascular endothelial growth factor. In conclusion, we show that GSC niches are present around arterioles and express bone marrow HSC niche proteins. |
Databáze: | OpenAIRE |
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