Generation of Med28 Specific Monoclonal Antibodies
| Autor: | Jong Hyuk Sung, Kwang Il Kim, Min A. Yu, Yeong Joon Jo, Ho Bin Yang, Sang Gyu Park, Jin Gu Cho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.drug_class
Immunology Blotting Western Freund's Adjuvant Gene Expression Breast Neoplasms Enzyme-Linked Immunosorbent Assay Monoclonal antibody Mice Antigen In vivo Antibody Specificity Antigens Neoplasm medicine Escherichia coli Immunology and Allergy Animals Humans Mice Inbred BALB C Hybridomas Mediator Complex biology Antibodies Monoclonal Original Articles Molecular biology Immunohistochemistry In vitro Recombinant Proteins Polyclonal antibodies Tumor progression biology.protein MCF-7 Cells Female Immunization Antibody HeLa Cells |
| Popis: | Med28 plays a role in transcription, signal transduction, and cell proliferation. The overexpression of med28 is associated with tumor progression in in vitro and in vivo models. Recently it has been reported that the elevated expression of med28 is associated with poor outcome in women with breast cancer. The expression level of med28 in in vitro and in vivo was examined by using anti-rabbit polyclonal antibody in previous reports. In this study, we report for the first time the generation and characterization of four monoclonal antibodies against med28 through immunoblotting, immunofluorescence microscopy, immunoprecipitation, and immunohistochemical analyses. These antibodies will be useful in detecting med28 in in vitro and in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|