Interleukin-6 does not stimulate bone resorption in neonatal mouse calvariae
| Autor: | Stuart H. Ralston, Karen Chapman, R. Graham G. Russell, D. E. Hughes, Lucien Aarden, Maxine Gowen, Abdul-Karim Al-Humidan |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Indomethacin Alpha (ethology) Parathyroid hormone Calvaria Bone resorption Mice Paracrine signalling Calcitriol Culture Techniques Internal medicine medicine Animals Orthopedics and Sports Medicine Bone Resorption Interleukin-6 business.industry Skull Drug Synergism Recombinant Proteins Resorption Endocrinology medicine.anatomical_structure Cytokine Animals Newborn Parathyroid Hormone business Interleukin-1 Hormone |
| Zdroj: | Journal of Bone and Mineral Research. 6:3-8 |
| ISSN: | 0884-0431 |
| Popis: | Recombinant human interleukin-6 (IL-6) was assessed for its ability to stimulate bone resorption in prelabeled mouse calvariae in vitro. IL-6 had no effect on bone resorption at concentrations ranging from 300 to 10,000 U/ml (3-1000 pg/ml). Neither the presence of indomethacin nor prolonged incubation periods (96 h) affected this result. IL-6 did not affect resorption stimulated by human recombinant IL-1 alpha (rIL-1 alpha) but inhibited resorption stimulated by parathyroid hormone (PTH) and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. rIL-1 alpha, PTH, and 1,25-(OH)2D3 induced IL-6 release by calvariae. We conclude from these studies that IL-6 does not stimulate bone resorption in neonatal mouse calvariae. However, it may act as a locally produced inhibitor and therefore a paracrine regulator of bone resorption induced by osteotropic hormones. IL-6 could also function as a long-range stimulator of systemic reactions and acute-phase responses to local inflammatory and neoplastic lesions in bone. |
| Databáze: | OpenAIRE |
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