Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
| Autor: | Koji Matsuura, Hiroshi Kano, Koyo Watanabe, Kotaro Nagata, Fukukazu Kawata, Jun Ning |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Microscope
Materials science ocis:(240.6680) Surface plasmons Surface plasmon Nanotechnology ocis:(180.4243) Near-field microscopy Adhesion Atomic and Molecular Physics and Optics law.invention Cell Studies law Microscopy ocis:(170.1530) Cell analysis Biophysics Fluorescence microscope Near-field scanning optical microscope Cell adhesion Biotechnology Localized surface plasmon |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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