Treatment with mononuclear cell populations improves post-infarction cardiac function but does not reduce arrhythmia susceptibility

Autor: Wilhelm Röll, Alexandra M. Klein, Bernd K. Fleischmann, Georg Nickenig, Thomas Beiert, Jan W. Schrickel, René Andrié, Alexander Ghanem, Markus Linhart, Florian Stöckigt, Vincent Knappe, Indra Lübkemeier
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Tachycardia
Epicardial Mapping
Cardiovascular Procedures
Cell Transplantation
Myocardial Infarction
030204 cardiovascular system & hematology
Ventricular Function
Left
Mice
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Myocyte
Myocytes
Cardiac
Myocardial infarction
Endothelial Progenitor Cells
Cardiomyocytes
Multidisciplinary
CD11b Antigen
Stem Cells
Scars
Heart
Cardiac Transplantation
Cardiovascular physiology
Infarction
Cardiology
cardiovascular system
Medicine
medicine.symptom
Stem cell
Anatomy
Cellular Types
Arrhythmia
Research Article
Cardiac function curve
medicine.medical_specialty
Science
Muscle Tissue
Surgical and Invasive Medical Procedures
Dermatology
03 medical and health sciences
Internal medicine
medicine
Animals
cardiovascular diseases
Progenitor cell
Transplantation
Muscle Cells
business.industry
Membrane Proteins
Biology and Life Sciences
Arrhythmias
Cardiac
Organ Transplantation
Cell Biology
medicine.disease
030104 developmental biology
Biological Tissue
Connexin 43
Leukocytes
Mononuclear
Tachycardia
Ventricular
Cardiovascular Anatomy
business
Zdroj: PLoS ONE, Vol 14, Iss 2, p e0208301 (2019)
PLoS ONE
ISSN: 1932-6203
Popis: BackgroundClinical and experimental data give evidence that transplantation of stem and progenitor cells in myocardial infarction could be beneficial, although the underlying mechanism has remained elusive. Ventricular tachyarrhythmia is the most frequent and potentially lethal complication of myocardial infarction, but the impact of mono nuclear cells on the incidence of ventricular arrhythmia is still not clear.ObjectiveWe aimed to characterize the influence of splenic mononuclear cell populations on ventricular arrhythmia after myocardial infarction.MethodsWe assessed electrical vulnerability in vivo in mice with left ventricular cryoinfarction 14 days after injury and intramyocardial injection of specific subpopulations of mononuclear cells (MNCs) (CD11b-positive cells, Sca-1-positive cells, early endothelial progenitor cells (eEPCs)). As positive control group we used embryonic cardiomyocytes (eCMs). Epicardial mapping was performed for analysing conduction velocities in the border zone. Left ventricular function was quantified by echocardiography and left heart catheterization.ResultsIn vivo pacing protocols induced ventricular tachycardia (VT) in 30% of non-infarcted mice. In contrast, monomorphic or polymorphic VT could be evoked in 94% of infarcted and vehicle-injected mice (pConclusionsTransplantation of different MNC populations after myocardial infarction improves left ventricular function similar to effects of eCMs. Prevention of inducible ventricular arrhythmia is only seen after transplantation of eCMs.
Databáze: OpenAIRE
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