Sodium Butyrate as a Histone Deacetylase Inhibitor Affects Toll-Like Receptor 4 Expression in Colorectal Cancer Cell Lines

Autor:	Mohammad Mahdi Mohammadi, Nazanin Atieh Kazemi Sefat, Saeed Talebi, Jamshid Hadjati, Hamid Daneshvar, Maryam Ajami
Rok vydání:	2019
Předmět:	0301 basic medicine Cell type Time Factors medicine.drug_class Immunology Gene Expression 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Gene expression medicine Humans Cytotoxic T cell MTT assay Receptor Dose-Response Relationship Drug Histone deacetylase inhibitor Sodium butyrate General Medicine HCT116 Cells Molecular biology digestive system diseases Histone Deacetylase Inhibitors Toll-Like Receptor 4 030104 developmental biology chemistry Cell culture 030220 oncology & carcinogenesis Butyric Acid Colorectal Neoplasms
Zdroj:	Immunological Investigations. 48:759-769
ISSN:	1532-4311 0882-0139
DOI:	10.1080/08820139.2019.1595643
Popis:	We assessed the effect of sodium butyrate (SB) as a histone deacetylase inhibitor (HDACi) on Toll-like receptor 4 (TLR4) gene expression levels, in low TLR4 expressing (HCT116) and high TLR4 expressing (SW480) colorectal cancer cells. The cytotoxic effect of SB was assessed by culturing SW480 and HCT116 cell lines using a broad spectrum of times and concentrations of SB. The MTT assay was done to check the cytotoxic properties of different SB concentrations. Gene expression levels of TLR4 was then evaluated for non-cytotoxic SB concentrations. Morphological analysis and MTT assay confirmed that SB concentrations equal to or less than 5mM were not cytotoxic for both cell lines. At 5mM concentration of SB in SW480 cell line and 1mM concentration of SB in HCT116 cell line, TLR4 gene expression level significantly increased from 24 to 48 hrs and decreased significantly from 48 to 72 hrs with an "early increased and late decreased pattern". At 1mM concentration of SB in SW480 cell line and 5mM concentration of SB in HCT116 cell line, TLR4 expression had a "gradually increased pattern". This study focuses on the dose-time-effect of SB in the pathogenesis of colorectal cancer. SB alters the expression level of TLR4 in colorectal cancer cells. This effect may depend on the cell type, treatment duration and SB concentration. The alterations in TLR4 expression may be due to the direct effect of SB on TLR4 and/or the expression changes of in other genes which may indirectly affect the TLR4 expression. Abbreviations: TLR4: Toll-like receptor 4; HDACi: histone deacetylase inhibitor; SB: sodium Butyrate; CRC: colorectal cancer; SCFA: short-chain fatty acid; hrs: hours.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::463aeb09649ebff2f892403fe2990022 https://doi.org/10.1080/08820139.2019.1595643 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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