In silico annotation of unreviewed acetylcholinesterase (AChE) in some lepidopteran insect pest species reveals the causes of insecticide resistance
| Autor: | Allah Ditta Abid, Najam ul Hassan, Mazhar Sadiq, Muhammad Saad Khan, Muhammad Sohail Shahzad, Azhar Abbas Khan, Ayesha Sarfaraz, Qudsia Yousafi, Umbreen Shahzad, Shahzad Saleem |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Signal peptide Physicochemical properties media_common.quotation_subject In silico Structural motif Insect Biology 01 natural sciences Lepidoptera genitalia 03 medical and health sciences chemistry.chemical_compound Exigua lcsh:QH301-705.5 media_common chemistry.chemical_classification Subcellular localization biology.organism_classification Acetylcholinesterase 030104 developmental biology Enzyme chemistry Biochemistry lcsh:Biology (General) Protein structure prediction Original Article Posttranslational modification General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Saudi Journal of Biological Sciences Saudi Journal of Biological Sciences, Vol 28, Iss 4, Pp 2197-2209 (2021) |
| ISSN: | 2213-7106 1319-562X |
| Popis: | Lepidoptera is the second most diverse insect order outnumbered only by the Coeleptera. Acetylcholinesterase (AChE) is the major target site for insecticides. Extensive use of insecticides, to inhibit the function of this enzyme, have resulted in the development of insecticide resistance. Complete knowledge of the target proteins is very important to know the cause of resistance. Computational annotation of insect acetylcholinesterase can be helpful for the characterization of this important protein. Acetylcholinesterase of fourteen lepidopteran insect pest species was annotated by using different bioinformatics tools. AChE in all the species was hydrophilic and thermostable. All the species showed lower values for instability index except L. orbonalis, S. exigua and T. absoluta. Highest percentage of Arg, Asp, Asn, Gln and Cys were recorded in P. rapae. High percentage of Cys and Gln might be reason for insecticide resistance development in P. rapae. Phylogenetic analysis revealed the AChE in T. absoluta, L. orbonalis and S. exigua are closely related and emerged from same primary branch. Three functional motifs were predicted in eleven species while only two were found in L. orbonalis, S. exigua and T. absoluta. AChE in eleven species followed secretory pathway and have signal peptides. No signal peptides were predicted for S. exigua, L. orbonalis and T. absoluta and follow non secretory pathway. Arginine methylation and cysteine palmotylation was found in all species except S. exigua, L. orbonalis and T. absoluta. Glycosylphosphatidylinositol (GPI) anchor was predicted in only nine species. |
| Databáze: | OpenAIRE |
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