Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts
| Autor: | Shinichi Abe, Koji Sakiyama, Katsuhide Kurokawa, Keiichi Ishigami, Tomotaka Takeda, Yoshinobu Ide |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Mrna expression
Stimulation macromolecular substances Biology General Biochemistry Genetics and Molecular Biology Cell Line Myoblasts Mice In vivo Myosin medicine Animals Myocyte RNA Messenger Muscle fibre Muscle Spindles Messenger RNA Myosin Heavy Chains Skeletal muscle General Medicine musculoskeletal system Molecular biology Cell biology medicine.anatomical_structure Stress Mechanical tissues |
| Zdroj: | Biomedical Research. 28:25-31 |
| ISSN: | 1880-313X 0388-6107 |
| DOI: | 10.2220/biomedres.28.25 |
| Popis: | In vivo studies have shown that changes in the characteristics of skeletal muscle fiber are determined by type of exercise or training. These earlier studies on mechanical stimulation, however, have all employed stimulation applied at a constant intensity, and no studies appear to have investigated change with variation of intensity of stimulation. In this study, we investigated the characteristics and differentiation of myoblasts stretched at different rates. Myoblasts were stimulated at 3 different rates, and the numbers of cells and nuclei on days 1, 3, and 5 were compared. The myosin heavy chain (MyHC) mRNA expression level was also compared. We investigated expression of MyHC-perinatal to determine speed of differentiation of myoblasts, and expression of MyHC-2b, 2d, and 2a to ascertain muscle cell characteristics. Counting cells and nuclei of myoblasts revealed clear promotion of differentiation with stretching. With rapid stretching, expression of MyHC-perinatal was high at first, but then showed a decrease. In terms of effect on muscle fiber characteristics, MyHC-2b, MyHC-2d, and MyHC-2a were high with rapid, medium, and slow stretching, respectively. This indicated that myoblast differentiation was promoted regardless of difference in stretching speed, with the myoblasts acquiring the muscle-fiber characteristics appropriate to each rate of stretching. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|