Characterisation of the Subgenomic RNAs of an Australian Isolate of Barley Yellow Dwarf Luteovirus
| Autor: | W. L. Gerlach, Peter M. Waterhouse, L. Kelly |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Gene Expression Regulation
Viral Genes Viral Transcription Genetic Molecular Sequence Data Luteovirus Gene product Transcription (biology) Virology Plant virus RNA Messenger Cloning Molecular Gene DNA Primers Subgenomic mRNA Viral Structural Proteins Genetics Base Sequence biology Australia RNA Hordeum biology.organism_classification Stop codon Protein Biosynthesis RNA Viral |
| Zdroj: | Virology. 202:565-573 |
| ISSN: | 0042-6822 |
| Popis: | We have characterised the subgenomic RNAs of an Australian isolate of BYDV-PAV. Northern blot analyses of infected plants and protoplasts have shown that this isolate synthesises three subgenomic RNAs. Precise mapping of the transcription start sites of all three subgenomic RNAs and translational analyses of subgenomic RNA 2 and 3 have revealed a number of features. First, the transcription start site of subgenomic RNA 1 in this isolate differs markedly from the start site determined for an Illinois isolate of BYDV-PAV. Second, the start sites of subgenomic RNA 1 and 2 occur at a sequence that closely resembles the 5′ end sequence of the genomic RNA (5′AGUGAAGA). Third, subgenomic RNA 2 appears to express ORF 6 of BYDV-PAV but the gene product is truncated due to the appearance of a new stop codon in the sequence. Last, subgenomic RNA 3, which is abundantly transcribed and encapsidated by the virus particle, appears to have no coding ability. We postulate that this novel subgenomic RNA has a regulatory function. |
| Databáze: | OpenAIRE |
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