Monolayer culture of a human pancreatic beta-cell adenoma
| Autor: | Marta Grinbergs, Vilma Lauris, J. Stuart Soeldner, Meng H. Tan, William L. Chick |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Adenoma
medicine.medical_specialty Pancreatic Beta Cell Adenoma Time Factors Endocrinology Diabetes and Metabolism medicine.medical_treatment Endocrinology Internal medicine Culture Techniques medicine Methods Humans Insulin Microscopy Phase-Contrast Beta (finance) Cells Cultured business.industry Monolayer culture Middle Aged medicine.disease Adenoma Islet Cell Culture Media Pancreatic Neoplasms Blood Glucose Cell culture Amylases Female Subculture (biology) business Fetal bovine serum |
| Zdroj: | Metabolism: clinical and experimental. 22(9) |
| ISSN: | 0026-0495 |
| Popis: | Monolayer cultures were prepared from a human beta-cell adenoma by dissociating the cells with a trypsin-collagenase solution. Cultures were grown in medium 199 containing 10% fetal bovine serum and 300 mg 100 ml glucose. They were maintained for a total of 2 mo and subcultured three times during this period. Insulin release from the primary cultures during the initial 24 days declined from approximately 150 to 40 mU per culture per 2 days, and then stabilized at this level prior to subculture on day 34. Subcultures became rapidly overgrown with fibroblastoid cells; after two subcultures, insulin release averaged approximately 1.5 mU per culture per 2 days. Reducing the glucose concentration in the medium from 300-100 mg 100 ml produced only a 7% reduction in insulin release, which conceivably helps to explain the high fasting insulin-to-glucose ratios observed in patients with these tumors. These data confirm that cell cultures derived from such tumors offer a unique model for studying human beta cells. |
| Databáze: | OpenAIRE |
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