Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction
| Autor: | Costantini, F., Tiggelaar, R. M., Salvio, R., Nardecchia, M., Schlautmann, S., Manetti, C., Gardeniers, H. J.G.E., Caputo, D., Nascetti, A., de Cesare, G., Baldini, Francesco, Siciliano, Pietro, Rossi, Marco, Scalise, Lorenzo, Di Natale, Corrado, Ferrari, Vittorio, Militello, Valeria, Miolo, Giorgia, Andò, Bruno, Marletta, Vincenzo, Marrazza, Giovanna |
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| Přispěvatelé: | Mesoscale Chemical Systems, MESA+ Institute |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Amorphous silicon
Materials science Microfluidics Settore CHIM/07 Photodetector anodic bonding microfluidics photosensors 02 engineering and technology Substrate (electronics) 01 natural sciences law.invention Horseradish peroxidase chemistry.chemical_compound Enzymatic reactions law Thin film Microchannel business.industry 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Photodiode chemistry Anodic bonding Photosensors Optoelectronics 0210 nano-technology business |
| Zdroj: | Lecture Notes in Electrical Engineering ISBN: 9783030043230 Sensors Sensors-Proceedings of the 4th National Conference on Sensors, 2018, 189-194 STARTPAGE=189;ENDPAGE=194;TITLE=Sensors-Proceedings of the 4th National Conference on Sensors, 2018 |
| ISSN: | 1876-1100 |
| Popis: | This work presents a portable lab-on-chip system, based on thin film electronic devices and an all-glass microfluidic network, for the real-time monitoring of enzymatic chemiluminescent reactions. The microfluidic network is patterned, through wet etching, in a 1.1 mm-thick glass substrate that is subsequently bonded to a 0.5 mm-thick glass substrate. The electronic devices are amorphous silicon p-i-n photosensors, deposited on the outer side of the thinner glass substrate. The photosensors, the microfluidic network and the electronic boards reading out the photodiodes’ current are enclosed in a small metallic box (\(10 \times 8 \times 15\,\mathrm{cm}^3\)) in order to ensure shielding from electromagnetic interferences. Preliminary tests have been performed immobilizing horseradish peroxidase on the inner wall of the microchannel as model enzyme for detecting hydrogen peroxide. Limits of detection and quantification equal to 18 and 60 \(\upmu \)M, respectively, have been found. These values are comparable to the best performances reported in literature for chemiluminescent-based optofluidic sensors. |
| Databáze: | OpenAIRE |
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