Role of mass spectrometry in the purification of peptides and proteins
| Autor: | Dorothy J. Phillips, Cecilia B. Mazza, Uwe D. Neue, Jie Y. Cavanaugh |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Chromatography Protein mass spectrometry Clinical Biochemistry Ion chromatography Proteins Peptide Fraction (chemistry) Cell Biology General Medicine Fractionation Reversed-phase chromatography Mass spectrometry Biochemistry Mass Spectrometry Sample preparation in mass spectrometry Analytical Chemistry chemistry Spectrophotometry Ultraviolet Peptides |
| Zdroj: | Journal of Chromatography B. 790:91-97 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/s1570-0232(03)00089-8 |
| Popis: | Experiments were carried out to evaluate the fractionation of proteins and peptides according to mass. Model mixtures were separated by either reversed-phase or ion-exchange chromatography with mass spectrometry-compatible mobile phase additives. Fraction collection was triggered by the mass/charge ratio of each one of the components of the mixture. Chromatography was additionally monitored with a UV-Vis detector in order to compare the new technique with generally accepted in separations. The results indicated that adequate purification is achieved by this new technique. Fraction collection triggered by changes in the mass/charge ratio reduces sample handling and analysis time. This study demonstrates the utility of mass-directed fractionation of peptides and proteins when mass spectrometry-compatible mobile phase additives are used. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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