In vitro and in vivo anti-Vibrio vulnificus activity of psammaplin A, a natural marine compound
Autor: | Jee H. Jung, Sang-Ho Choi, Byung Cheol Lee, Arim Lee, Tae Sung Kim |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Aquatic Organisms Cancer Research Spleen Microbial Sensitivity Tests Vibrio vulnificus 01 natural sciences Biochemistry Cell Line Microbiology Mice 03 medical and health sciences In vivo Vibrio Infections Genetics medicine Animals Humans Disulfides Cytotoxicity Molecular Biology Biological Products Molecular Structure integumentary system biology 010405 organic chemistry fungi Epithelial Cells equipment and supplies bacterial infections and mycoses biology.organism_classification Antimicrobial In vitro Anti-Bacterial Agents 0104 chemical sciences Disease Models Animal 030104 developmental biology medicine.anatomical_structure Oncology Cell culture Tyrosine bacteria Molecular Medicine Female |
Zdroj: | Molecular Medicine Reports. 14:2691-2696 |
ISSN: | 1791-3004 1791-2997 |
Popis: | Vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. In the present study, the antimicrobial activity of natural marine product-derived compounds against V. vulnificus, were investigated in vitro and in vivo. Twelve pure compounds were isolated from natural marine products and their inhibitory effects on V. vulnificus-induced cytotoxicity were determined in INT‑407 cells. Among the 12 pure compounds tested, treatment with psammaplin A significantly suppressed V. vulnificus‑induced cytotoxicity in INT‑407 cells. Notably, treatment with psammaplin A (5-50 µg) had improved survival rates compared with that in the untreated mice, when the mice were infected with V. vulnificus intraperitoneally. In addition, the bacterial load of V. vulnificus in several tissues (spleen, liver and small intestine) was significantly lower in psammaplin A‑treated mice than in untreated mice. Furthermore, psammaplin A treatment significantly suppressed the growth of V. vulnificus. Taken together, these results indicate that psammaplin A may be a potential agent for the prevention and treatment of V. vulnificus infections. |
Databáze: | OpenAIRE |
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