Quantification and Patterns of Endothelial Cell Loss Due to Eye Bank Preparation and Injector Method in Descemet Membrane Endothelial Keratoplasty Tissues
| Autor: | Christopher G. Stoeger, Jeffrey D. Holiman, Winston Chamberlain, Julie M. Schallhorn |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Endothelium Descemet membrane Cell Survival medicine.medical_treatment Cell Count Eye Banks law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Ophthalmology medicine Humans Fluorescein Descemet Membrane Corneal transplantation Aged business.industry Endothelium Corneal Eye bank Injector Corneal Endothelial Cell Loss Middle Aged Fluoresceins Tissue Donors Surgery Endothelial stem cell medicine.anatomical_structure chemistry Descemet Stripping Endothelial Keratoplasty 030221 ophthalmology & optometry Tissue and Organ Harvesting Female business 030217 neurology & neurosurgery |
| Zdroj: | Cornea. 35(3) |
| ISSN: | 1536-4798 |
| Popis: | Purpose To evaluate endothelial cell damage after eye bank preparation and passage through 1 of 2 different injectors for Descemet membrane endothelial keratoplasty grafts. Methods Eighteen Descemet membrane endothelial keratoplasty grafts were prepared by Lions VisionGift with the standard partial prepeel technique and placement of an S-stamp for orientation. The grafts were randomly assigned to injection with either a glass-modified Jones tube injector (Gunther Weiss Scientific Glass) or a closed-system intraocular lens injector (Viscoject 2.2; Medicel). After injection, the grafts were stained with the vital fluorescent dye Calcein AM and digitally imaged. The percentage of cell loss was calculated by measuring the area of nonfluorescent pixels and dividing it by the total graft area pixels. Results Grafts injected using the modified Jones tube injector had an overall cell loss of 27% ± 5% [95% confidence interval, 21%-35%]. Grafts injected using the closed-system intraocular lens injector had a cell loss of 32% ± 8% (95% confidence interval, 21%-45%). This difference was not statistically significant (P = 0.3). Several damage patterns including damage due to S-stamp placement were observed, but they did not correlate with injector type. Conclusions In this in vitro study, there was no difference in the cell loss associated with the injector method. Grafts in both groups sustained significant cell loss and displayed evidence of graft preparation and S-stamp placement. Improvement in graft preparation and injection methods may improve cell retention. |
| Databáze: | OpenAIRE |
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