Improved control of black rot of broccoli caused by Xanthomonas campestris pv. campestris using a bacteriophage and a nonpathogenic Xanthomonas sp. strain
| Autor: | Daisuke Maekawa, Noriyuki Miyake, Hirofumi Nagai, Yuichi Takikawa, Yasuhiro Inoue, Shinro Kato |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Strain (chemistry) Wettable powder Inoculation Biological pest control food and beverages Plant Science biology.organism_classification 01 natural sciences Xanthomonas campestris Microbiology Xanthomonas campestris pv. campestris Bacteriophage 03 medical and health sciences Xanthomonas sp Horticulture 030104 developmental biology Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | SC10201804160192 NARO成果DBd C10201801310004_3392.pdf |
| ISSN: | 1610-739X 1345-2630 |
| DOI: | 10.1007/s10327-017-0745-4 |
| Popis: | We examined the potential for biological control of black rot of broccoli, caused by Xanthomonas campestris pv. campestris (Xcc), using nonpathogenic Xanthomonas sp. strain 11-100-01 (npX) mixed with bacteriophage XcpSFC211 (pXS). Inoculation of intact broccoli plants in greenhouse trials with either npX or pXS did not control black rot. After injured plant inoculation, however, npX alone or npX with pXS significantly controlled black rot. When a mixed suspension of npX with pXS was placed on a membrane filter, then washed with distilled water and air-dried, a substantial amount of pXS adsorbed to the surface of npX. In a field trial, broccoli plants were sprayed with a suspension of npX with pXS, then inoculated with Xcc. A meta-analysis of the results from five field trials showed an integrated risk ratio (IRR, the ratio of disease incidence in inoculated broccoli plants to the incidence in control plants) of 0.69 after treatment with only npX and 0.59 with npX with pXS, indicating that black rot incidence was significantly reduced by each treatment. The difference between these two treatments was also significant. IRR was 1.24 when comparing suppression by npX with pXS and that by basic copper sulfate wettable powder; thus, their control was comparable. The combination of npX with pXS improved the preventive effect against black rot. This is the first report describing that a nonpathogenic Xanthomonas sp. strain mixed with a bacteriophage effectively controlled black rot of broccoli in field trials. |
| Databáze: | OpenAIRE |
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