Evaluation of N,N-diethyl-m-toluamide (DEET) as a topical agent for preventing skin penetration by cercariae of Schistosoma mansoni
| Autor: | Takeshi Shibuya, Diane Nowicki, Yi Xun He, B. Salafsky, Gary Anderson, Kalyanasundaram Ramaswamy |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Administration Topical DEET Schistosomiasis Pharmacology Mice chemistry.chemical_compound In vivo Virology parasitic diseases medicine Animals Skin biology Schistosoma mansoni Viral tegument medicine.disease biology.organism_classification Schistosomiasis mansoni In vitro Disease Models Animal Microscopy Electron Infectious Diseases chemistry Insect Repellents Immunology Parasitology Trematoda Insect repellent |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 58:828-834 |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.1998.58.828 |
| Popis: | The effect of skin application of N,N-diethyl-m-toluamide (DEET) on the penetration and migration behavior of cercariae of Schistosoma mansoni was evaluated in vitro and in vivo in a mouse model. These studies showed that DEET at concentrations of 7.5% or higher was 100% effective in immobilizing and killing cercariae of S. mansoni in vitro. Ultrastructural studies on such DEET-exposed cercariae showed transformative and degenerative changes involving both tegument and deeper parenchymal structures. Fatal tissue lesions were evident as early as 5 min postexposure to DEET, and became more extensive with increasing exposure time. Cutaneous application of DEET (as a pure chemical in isopropanol or as a commercial insect repellent preparation) was more than 99% effective in preventing entry of S. mansoni cercariae into the mouse tail skin. Radiolabeling and tracer studies confirmed that 7.5% DEET applied to the skin prior to infection was highly effective in preventing schistosomular migration to the lungs. |
| Databáze: | OpenAIRE |
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