Role of Epigenetic Regulation by the REST/CoREST/HDAC Corepressor Complex of ModerateNANOGExpression in Chicken Primordial Germ Cells
| Autor: | Young Hwang, Deivendran Rengaraj, Hyun Gyo Jung, Jae Yong Han, Young Hyun Park, Ho Yeon Cho |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Homeobox protein NANOG endocrine system RE1-silencing transcription factor Histone Deacetylases Epigenesis Genetic Avian Proteins Histones 03 medical and health sciences Histone H3 0302 clinical medicine Animals Epigenetics Cells Cultured Embryonic Stem Cells reproductive and urinary physiology biology fungi Gene Expression Regulation Developmental Acetylation Nanog Homeobox Protein Cell Biology Hematology Spermatogonia Cell biology Repressor Proteins RCOR1 Germ Cells 030104 developmental biology Histone 030220 oncology & carcinogenesis embryonic structures biology.protein Histone deacetylase biological phenomena cell phenomena and immunity Chickens Co-Repressor Proteins Corepressor Developmental Biology |
| Zdroj: | Stem Cells and Development. 27:1215-1225 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2018.0059 |
| Popis: | Primordial germ cells (PGCs), the precursors of gametes, have regulatory mechanisms involving transcription factors and epigenetic modifications. The transcription factor NANOG is a key regulator of germ cell and embryonic stem cell characteristics. However, the epigenetic regulation of NANOG with a histone deacetylase (HDAC) complex in PGCs has not been studied. In this study, we investigated the epigenetic regulation, and in particular the histone acetylation, of NANOG in chicken PGCs. Intriguingly, although NANOG was highly activated in chicken PGCs, the upstream region of its promoter was moderately suppressed by histone deacetylation. HDAC inhibition induced histone H3 lysine 9 acetylation (H3K9ac) and derepressed NANOG expression. Furthermore, knockdown studies revealed that HDAC complex members, such as RE1-silencing transcription factor (REST) and REST corepressor 3 (RCOR3), are important epigenetic modulators of NANOG expression in chicken PGCs. We demonstrate that moderate regulation of NANOG by the REST/CoREST/HDAC complex might be crucial for maintaining the integrity of PGCs. |
| Databáze: | OpenAIRE |
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