Absence of Mutations in the CDKN2 Binding Site of CDK4 in Childhood Acute Lymphoblastic Leukemia
| Autor: | Birgit Beyermann, Günter Henze, Anja Möricke, Joachim Köchling, Tillmann Taube, Christian Kebelmann-betzig, Hagen Graf v. Einsiedel, Serge Dragon, Karlheinz Seeger |
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| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
DNA Mutational Analysis Biology Exon Bone Marrow Cyclin-dependent kinase Proto-Oncogene Proteins Tumor Cells Cultured Humans Point Mutation Genetic Testing Binding site Child Gene Childhood Acute Lymphoblastic Leukemia Cyclin-Dependent Kinase Inhibitor p16 Binding Sites Point mutation Retinoblastoma protein Cyclin-Dependent Kinase 4 Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma Cell cycle Molecular biology Cyclin-Dependent Kinases Oncology Calibration biology.protein Cancer research |
| Zdroj: | Leukemia & Lymphoma. 40:413-417 |
| ISSN: | 1029-2403 1042-8194 |
| DOI: | 10.3109/10428190109057941 |
| Popis: | The cell cycle regulatory circuit resulting in phosphorylation of the retinoblastoma protein (pRB) is frequently altered in human cancers. Several mechanisms of disruption are known in that pathway. In childhood acute lymphoblastic leukemia (ALL), the main disrupting mechanism is the homozygous deletion of the CDKN2 (cyclin dependent kinase inhibitor 2) genes: p16CDKN2a, p15CDKN2b, and p19ARF. Another pRB pathway disturbance is a previously described point mutation in the exon 2 of CDK4, a pRB phosphorylating enzyme, which abrogates binding of the latter to its inhibitors, p16CDKN2a and p15CDKN2b. Here we report the absence of point mutations in the CDKN2-binding site of CDK4 in 100 cases of childhood ALL, 2 cases of childhood chronic myeloid leukemia and 9 hematologic cell lines screened by PCR-SSCP (polymerase chain reaction single stranded conformational polymorphism gel electrophoresis), thereby minimizing the possibility of the existence of these specific CDK4 mutations in childhood ALL. |
| Databáze: | OpenAIRE |
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