First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid
| Autor: | Stefano Menzo, Martina Grilli, Francesca Gonnelli, Martina Bonifazi, Lina Zuccatosta, Federico Mei, Maria Agnese Latini, Michele Sediari, Alessandro Di Marco Berardino, Stefano Gasparini, Luca Paolini, Claudia Duranti, Giacomo Spurio Vennarucci, Antonina Re |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
safety
Male Pulmonary and Respiratory Medicine ARDS COVID-19 Vaccines Pleural effusion medicine.medical_treatment Pneumonia Viral Thoracentesis Real-Time Polymerase Chain Reaction medicine.disease_cause Critical Care and Intensive Care Medicine Article Betacoronavirus 03 medical and health sciences COVID-19 Testing 0302 clinical medicine medicine Humans 030212 general & internal medicine TUS thoracic ultrasound Pandemics COVID-19 coronavirus disease 2019 Aged Coronavirus RT-PCR real-time reverse-transcriptase–polymerase-chain-reaction Clinical Laboratory Techniques Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 business.industry computed tomography-guided biopsy COVID-19 Gold standard (test) medicine.disease Virology Reverse transcriptase CT computed tomography ultrasound-guided biopsy meta-analysis Pleural Effusion Real-time polymerase chain reaction 030228 respiratory system diagnostic yield Coronavirus Infections business Cardiology and Cardiovascular Medicine Asymptomatic carrier pleural lesions |
| Zdroj: | Chest |
| ISSN: | 0012-3692 |
| DOI: | 10.1016/j.chest.2020.05.583 |
| Popis: | Coronavirus disease 2019 (COVID-19) is a pandemic infection due to the spread of a novel coronavirus (severe acute respiratory syndrome coronavirus 2), resulting in a wide range of clinical features, from asymptomatic carriers to ARDS. The gold standard for diagnosis is nucleic acid detection by real-time reverse transcriptase-polymerase chain reaction in nasopharyngeal swabs. However, due to limitations in this technique's sensitivity, thoracic imaging plays a crucial, complementary role in diagnostic evaluation and also allows for detection of atypical findings and potential alternative targets for sampling (eg, pleural effusion). Although less common, pleural involvement has been described in a minority of patients. This report describes the first case of reverse transcriptase-polymerase chain reaction detection of severe acute respiratory syndrome coronavirus 2 in pleural fluid obtained by means of ultrasound-guided thoracentesis, and its main characteristics are detailed. Pleural effusion is not a common finding in COVID-19 infection, but a prompt recognition of this potential localization may be useful to optimize diagnostic evaluation as well as the management of these patients. |
| Databáze: | OpenAIRE |
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