A Bioaccumulative Cyclometalated Platinum(II) Complex with Two-Photon-Induced Emission for Live Cell Imaging
| Autor: | Yun Wah Lam, Kok Wai Cheah, Ka-Leung Wong, Chi Kin Koo, Yangyi Yang, Leo K.Y. So, Jin Can Chen, Cornelia Wing Yin Man, Kai-Chung Lau, Hoi Lam Tam, Sai Wah Tsao, Michael H.W. Lam |
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| Rok vydání: | 2009 |
| Předmět: |
Cell Survival
Photochemistry chemistry.chemical_element Platinum Compounds Cell Line law.invention Inorganic Chemistry HeLa Mice chemistry.chemical_compound Two-photon excitation microscopy Confocal microscopy law Live cell imaging Pyridine Animals Humans Physical and Theoretical Chemistry Photons Molecular Structure biology Ligand biology.organism_classification chemistry Cyclization Spectrophotometry Platinum Luminescence |
| Zdroj: | Inorganic Chemistry. 48:872-878 |
| ISSN: | 1520-510X 0020-1669 |
| Popis: | The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C(phenyl), N(pyridyl), and N(pyrazolyl) donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. |
| Databáze: | OpenAIRE |
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