Inositides in nuclei of Friend cells: changes of polyphosphoinositide and diacylglycerol levels accompany cell differentiation
| Autor: | Lucia Manzoli, Silvia Rubbini, R S Gilmour, Anna Maria Billi, Amelia Cataldi, Lucio Cocco, Alberto M. Martelli |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Phosphatidylinositol 4
5-Diphosphate Cellular differentiation Phosphatidylinositol Phosphates Tumor cells Biology Friend Murine Leukemia Virus Phosphatidylinositols Diglycerides Mice Pi medicine Tumor Cells Cultured Animals Diacylglycerol kinase Cell Nucleus Leukemia Experimental Cell Differentiation Cell Biology Cell Transformation Viral Cell biology Friend murine leukemia virus Cell nucleus medicine.anatomical_structure Biochemistry lipids (amino acids peptides and proteins) Leukemia Erythroblastic Acute Nucleus |
| Zdroj: | Cellular signalling. 7(1) |
| ISSN: | 0898-6568 |
| Popis: | Friend erythroleukemia cells were labelled with high levels of [3H]myo-inositol and the radioactivity in PI, PIP and PIP2, extracted from isolated nuclei, was measured. A parallel analysis employing a picomole sensitive assay for both PIP and DAG has been carried out. The results indicate that the differentiation process is characterised by an accumulation of nuclear PIP and PIP2 and by a decrease of DAG mass. We suggest that as differentiation proceeds toward erythrocytes in Friend cells, this is accompanied by a reduction in the amount of these messengers in the nucleus. |
| Databáze: | OpenAIRE |
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