Separation of anti-dinitrophenyl antibodies according to affinity by fractionated elution from immunoadsorbent using thiocyanate
| Autor: | H. Mossmann, A. Vogt, F. Shimizu, H. Takamiya, K. Himmelspach |
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| Rok vydání: | 1978 |
| Předmět: |
Molar concentration
chemical and pharmacologic phenomena Antibodies chemistry.chemical_compound Column chromatography Stepwise elution Animals Humans Serum Albumin Chromatography Thiocyanate biology Elution General Medicine chemistry biology.protein Immunologic Techniques Dinitrophenyl Cattle Binding Sites Antibody gamma-Globulins Antibody Carrier Proteins Hapten Dinitrophenols Thiocyanates |
| Zdroj: | Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie. 173(2) |
| ISSN: | 0300-9130 |
| Popis: | The possibility of fractionating anti-DNP-antibodies according to their affinity by successive elution from a DNP-immunoadsorbent with increasing concentrations of thiocyanate (KSCN) was investigated. Using KSCN at molarities of 0.5 M, 2.0 M and 3.0 M in a stepwise elution procedure, the mean affinity of the eluted antibody fractions was found to increase with increasing molarity of the eluent. The method described is a simple, reliable means of separating antibodies on an affinity basis which is of special value in systems where haptens are not available. |
| Databáze: | OpenAIRE |
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