P2Y Receptor Modulation of ATP Release in the Urothelium
| Autor: | Kylie J Mansfield, Jessica R. Hughes |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Agonist
Adenosine monophosphate medicine.medical_specialty P2Y receptor Article Subject medicine.drug_class lcsh:Medicine Uridine Triphosphate Hypotonic response Biology General Biochemistry Genetics and Molecular Biology Cell Line chemistry.chemical_compound Adenosine Triphosphate Internal medicine medicine Humans Adenosine Triphosphatases General Immunology and Microbiology lcsh:R Purinergic receptor General Medicine Purinergic signalling Adenosine Monophosphate Cell biology Adenosine Diphosphate Adenosine diphosphate Endocrinology chemistry Receptors Purinergic P2Y Urothelium Adenosine triphosphate Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2014 (2014) |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2014/830374 |
| Popis: | The release of ATP from the urothelium in response to stretch during filling demonstrates the importance of the purinergic system for the physiological functioning of the bladder. This study examined the effect of P2 receptor agonists on ATP release from two urothelial cell lines (RT4 and UROtsa cells). Hypotonic Krebs was used as a stretch stimulus. Incubation of urothelial cells with high concentrations of the P2Y agonist ADP induced ATP release to a level that was 40-fold greater than hypotonic-stimulated ATP release (P< 0.0011, ADP EC50 1.8 µM). Similarly, an increase in ATP release was also observed with the P2Y agonist, UTP, up to a maximum of 70% of the hypotonic response (EC50 0.62 µM). Selective P2 receptor agonists,αβ-methylene-ATP, ATP-γ-S, and 2-methylthio-ADP had minimal effects on ATP release. ADP-stimulated ATP release was significantly inhibited by suramin (100 µM,P= 0.002). RT4 urothelial cells break down nucleotides (100 µM) including ATP, ADP, and UTP to liberate phosphate. Phosphate liberation was also demonstrated from endogenous nucleotides with approximately 10% of the released ATP broken down during the incubation. These studies demonstrate a role for P2Y receptor activation in stimulation of ATP release and emphasize the complexity of urothelial P2 receptor signalling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|