Dietary omega-3 fatty acid supplementation does not impair vitamin E status or promote lipid peroxidation in growing horses
| Autor: | J. Kivipelto, Lori K. Warren, Sarah H White-Springer, Kelly R. Vineyard |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
030309 nutrition & dietetics medicine.medical_treatment Non Ruminant Nutrition Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Fish Oils 0302 clinical medicine Animal science Fatty Acids Omega-3 Genetics medicine Animals Vitamin E Horses chemistry.chemical_classification 0303 health sciences Cholesterol Glutathione peroxidase 030229 sport sciences General Medicine Fish oil Eicosapentaenoic acid chemistry Docosahexaenoic acid Dietary Supplements Animal Science and Zoology Lipid Peroxidation Food Science |
| Zdroj: | J Anim Sci |
| ISSN: | 1525-3163 0021-8812 |
| Popis: | Omega-3 (n-3; ω-3) fatty acids (FA) are often included in the diet for their potential health benefits. However, because oxidative potential is increased with the degree of unsaturation in vitro, polyunsaturated FA such as eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) may be at increased risk of lipid peroxidation. We aimed to determine the effects of dietary n-3 FA supplementation on antioxidant status and lipid peroxidation in yearling horses. Quarter Horses (mean ± SEM; 14.6 ± 0.2 mo) were randomly assigned to receive no n-3 FA supplementation (CON; n = 6) or 60 mg n-3/kg body weight from milled flaxseed (FLAX; n = 6) or encapsulated fish oil (FISH; n = 6). All horses received a basal diet of mixed grain concentrate fed individually at 1.5% body weight (dry matter basis) and ad libitum bahiagrass pasture forage. Blood samples were obtained before and after 70 d of supplementation to evaluate vitamin E, selenium, lipids, antioxidant status, and oxidative stress. Data were analyzed using a mixed model ANOVA with repeated measures. Supplementation with n-3 FA did not reduce serum vitamin E or Se and, in fact, elevated (P ≤ 0.0003) vitamin E status in FISH horses. At day 70, serum triglycerides were lower in FISH and FLAX horses than CON horses (P ≤ 0.02) and F2-isoprostanes were lower in FISH than CON horses (P = 0.0002). Dietary n-3 FA had no effect on cholesterol, reduced and oxidized glutathione, glutathione peroxidase, and thiobarbituric acid-reactive substances. In growing horses fed to meet their vitamin E requirements, supplementation with 60 mg n-3/kg body weight did not negatively affect vitamin E status or promote lipid peroxidation. Elevated vitamin E status in horses fed FISH, coupled with lower serum F2-isoprostanes, further suggest that the longer-chain, highly unsaturated n-3 FA, EPA and DHA, may actually attenuate lipid peroxidation. |
| Databáze: | OpenAIRE |
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