Novel nanocarrier for oral Hepatitis B vaccine
| Autor: | Madhusudan Bhat, Sarat Kumar Kottarath, Sandeep Kumar Srivastava, C.K. Prashant, Amit K. Dinda |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
HBsAg Hepatitis B vaccine Administration Oral Biocompatible Materials 02 engineering and technology Booster dose Poloxamer Immunoglobulin G 03 medical and health sciences Mice Antigen Oral administration Medicine Animals Hepatitis B Vaccines Seroconversion Hepatitis B Antibodies Drug Carriers Hepatitis B Surface Antigens General Veterinary General Immunology and Microbiology biology business.industry Macrophages Vaccination Public Health Environmental and Occupational Health 021001 nanoscience & nanotechnology Hepatitis B Immunoglobulin A 030104 developmental biology Infectious Diseases Immunology Antibody Formation biology.protein Molecular Medicine Nanoparticles Female Antibody 0210 nano-technology business |
| Zdroj: | Vaccine. 34(27) |
| ISSN: | 1873-2518 |
| Popis: | Oral vaccination is a safe, cost effective and non-invasive method suitable for mass immunization. We fabricated nanoparticle (NP) with 14kd polycaprolactone (PCL) entrapping hepatitis B surface antigen (HBsAg) stabilized with Pluronics® F127 and used it as oral delivery vehicle. We evaluated its efficacy for specific antibody production and compared with parenteral routes of immunization in mice. We found a superior antibody response with a higher titer of anti-HBsAg antibody till 2 months following single oral administration compared to other routes of immunization and conventional alum-based HBsAg vaccine. The NPs with the antigen were found in the macrophages in small intestinal villi, peripheral lymph nodes and other reticulo-endothelial organs 2 months after oral administration. This study suggests the efficacy of the current nanocarrier system for efficient antigen presentation disseminated in peripheral lymphoid tissues following oral administration with a prolonged antibody response, which can minimize the requirement of booster dose. |
| Databáze: | OpenAIRE |
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