Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
| Autor: | Marcelo U. Ferreira, Hernando A. del Portillo, Luiz Marcelo Aranha Camargo, G Levitus, Frédéric Mertens |
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| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Male
GST-recombinant clones Plasmodium vivax Genes Protozoan Protozoan Proteins lcsh:QR1-502 Antibodies Protozoan Polymerase Chain Reaction lcsh:Microbiology law.invention law Antibody Specificity protein immunoblotting Cloning Molecular Malaria Falciparum chemistry.chemical_classification biology Protein primary structure Malária merozoite surface protein 1 Amino acid Amazônia Protozoário Recombinant DNA human IgG Female ELISA Antibody Brazil Microbiology (medical) lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Recombinant Fusion Proteins Blotting Western Plasmodium falciparum DNA Recombinant Antigens Protozoan Enzyme-Linked Immunosorbent Assay Antigen Species Specificity parasitic diseases medicine Malaria Vivax vivax malaria Animals Humans Chagas Disease Protein Precursors Gene medicine.disease biology.organism_classification Virology Molecular biology chemistry Immunoglobulin G biology.protein Uruguay Malaria |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 87, Pp 77-84 (1992) Repositório Institucional da UnB Universidade de Brasília (UnB) instacron:UNB Memórias do Instituto Oswaldo Cruz, Volume: 87 Supplement 3, Pages: 77-84, Published: 1992 |
| ISSN: | 1678-8060 0074-0276 |
| Popis: | The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections. _______________________________________________________________________________ RESUMO A estrutura primária completa do gene que codifica a proteína de superfície Merozoite 1 de Plasmodium vivax (PvMSP-1) revelou a existência de regiões conservadas entre espécies entre os análogos de proteínas de outras espécies de plasmódios. Aqui, três clones de DNA recombinante expressando 50, 200 e 500 aminoácidos da região N-terminal da proteína-1 PvMSP foram utilizados em ensaios de ELISA e immunoblotting proteína de olhar para as respostas de anticorpos IgG de pacientes com malária da Amazônia Brasileira de Rondônia. Os resultados mostraram a existência de P. vivax e P. falciparum anticorpos IgG dirigidos contra PvMSP-1 determinantes antigênicos expressos nos clones contendo os primeiros 200 e os 500 seguintes aminoácidos da molécula, mas não dentro da uma expressão mais N- terminal de 50 aminoácidos. Curiosamente, não houve correlação entre os níveis destes anticorpos IgG e no número anterior de malária. |
| Databáze: | OpenAIRE |
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