A Metalloprotease Secreted by the Insect Pathogen Photorhabdus luminescens Induces Melanization
| Autor: | Celeste A. Berg, Christopher N. LaRock, David A. D'Argenio, Carleen M. Collins, Kiara Held |
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| Rok vydání: | 2007 |
| Předmět: |
animal structures
Blotting Western Molecular Sequence Data Applied Microbiology and Biotechnology Microbiology Bacterial Proteins Manduca Photorhabdus luminescens Invertebrate Microbiology Animals Amino Acid Sequence Pathogen Melanins Innate immune system Ecology biology Reverse Transcriptase Polymerase Chain Reaction fungi biology.organism_classification Molecular biology Recombinant Proteins Galleria mellonella Secretory protein Manduca sexta Culture Media Conditioned Larva Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Metalloproteases Electrophoresis Polyacrylamide Gel Photorhabdus Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 73:7622-7628 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Photorhabdus luminescens is a gram-negative insect pathogen that enters the hemocoel of infected hosts and produces a number of secreted proteins that promote colonization and subsequent death of the insect. In initial studies to determine the exact role of individual secreted proteins in insect pathogenesis, concentrated culture supernatants from various P. luminescens strains were injected into the tobacco hornworm Manduca sexta . Culture supernatants from P. luminescens TT01, the genome-sequenced strain, stimulated a rapid melanization reaction in M. sexta . Comparison of the profiles of secreted proteins from the various Photorhabdus strains revealed a single protein of approximately 37 kDa that was significantly overrepresented in the TT01 culture supernatant. This protein was purified by DEAE ion-exchange and Superdex 75 gel filtration chromatography and identified by matrix-assisted laser desorption ionization-time of flight analysis as the product of the TT01 gene plu1382 (NCBI accession number NC_005126); we refer to it here as PrtS. PrtS is a member of the M4 metalloprotease family. Injection of PrtS into larvae of M. sexta and Galleria mellonella and into adult Drosophila melanogaster and D. melanogaster melanization mutants ( Bc ) confirmed that the purified protein induced the melanization reaction. The prtS gene was transcribed by P. luminescens injected into M. sexta before death of the insect, suggesting that the protein was produced during infection. The exact function of this protease during infection is not clear. The bacteria might survive inside the insect despite the melanization process, or it might be that the bacterium is specifically activating melanization in an attempt to circumvent this innate immune response. |
| Databáze: | OpenAIRE |
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