Overexpression of Human Chorionic Gonadotropin β Genes 3, 5 and 8 in Tumor Tissue and Urinary Cells of Bladder Cancer Patients
| Autor: | Erkki Rintala, Kristina Hotakainen, Ulf-Håkan Stenman, Riikka Järvinen, Susanna Lintula, Annukka Paju, Jakob Stenman |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male endocrine system medicine.medical_specialty Urinary system Trophoblastic Tumor Urine Human chorionic gonadotropin Internal medicine Biomarkers Tumor medicine Humans Chorionic Gonadotropin beta Subunit Human RNA Messenger Gene reproductive and urinary physiology Aged Aged 80 and over Bladder cancer Reverse Transcriptase Polymerase Chain Reaction urogenital system business.industry Reproducibility of Results DNA Neoplasm General Medicine Middle Aged Serum concentration Prognosis medicine.disease Tumor tissue Gene Expression Regulation Neoplastic Endocrinology Real-time polymerase chain reaction Urinary Bladder Neoplasms embryonic structures Cancer research Female business hormones hormone substitutes and hormone antagonists Genes Neoplasm |
| Zdroj: | Tumor Biology. 28:52-56 |
| ISSN: | 1423-0380 1010-4283 |
| Popis: | Human chorionic gonadotropin (hCG) is a marker of trophoblastic tumors, and the serum concentration of the free beta-subunit (hCGbeta) is an independent prognostic marker in several nontrophoblastic cancers. hCGbeta is encoded by six genes, of which the type II genes (hCGbeta 3/9, 5 and 8) are thought to be upregulated in relation to type I genes (hCGbeta 6/7) in cancer.We developed a novel quantitative RT-PCR method for the quantification of the relative expression levels of the two groups of hCGbeta genes and analyzed 28 bladder tumors and 15 urine samples.We found a higher relative expression level of type II genes in malignant compared with benign urothelia (p = 0.016) and in exfoliated urinary cells from cancer patients compared with those from benign controls (p = 0.026). The expression level was increasing with higher stage (p = 0.014) and grade (p = 0.001) and tended to be higher in relapsing tumors (p = 0.059).The increased hCGbeta concentrations in body fluids of patients with aggressive bladder cancer may be due to overexpression of type II genes. Quantification of the relative mRNA expression levels of the hCGbeta type I and II genes in urine cells should be further studied as a potential noninvasive tool for the diagnosis and follow-up of bladder cancer. |
| Databáze: | OpenAIRE |
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